Adam S
Int J Radiat Biol Relat Stud Phys Chem Med. 1982 Nov;42(5):531-44. doi: 10.1080/09553008214551471.
gamma-Radiolysis (dose-rate: 0 X 89 Gy/s) or electron (e)-radiolysis (dose-rate: 5 X 10(7) Gy/s) of unbuffered aqueous solutions of alpha, alpha-trehalose (concentration: 60 mg/ml, radiation dose: 20 kGy) at 0 degree C yielded glucose (Ggamma = 1 X 7; Ge = 0 X 63) and 5-deoxyxylohexodialdose (Ggamma = 0 X 21; Ge = 0 X 05). Buffering at pH-values of 5 X 0 or 5 X 5 and irradiation caused increased formation of these monomeric products, particularly of the deoxy-compound. On addition of increasing amounts of bovine serum albumin or ovalbumin (10-30 mg/ml) and irradiation the yields of products were markedly reduced. The decrease in glucose formation was less pronounced when sperm whale myoglobin was present during gamma- or electron-irradiation. The G-values of 5-deoxyxylohexodialdose, however, were increased by 45 per cent (gamma-irradiation) and 70 per cent (electron-irradiation) at approximately 10 mg/ml of admixed myoglobin. Further increase in myoglobin concentration led to a gradual decrease in the yields of the deoxy-product. The observed effects are explained by scavenging of water radicals and by interactions of the added substrates with sugar radicals. Emulsified lipids (palmitic acid methylester or trilinoleic glycerol) did not affect the radiation-induced formation of products from trehalose.
在0℃下，对浓度为60mg/ml、辐射剂量为20kGy的α,α-海藻糖无缓冲水溶液进行γ辐射分解（剂量率：0×89Gy/s）或电子（e）辐射分解（剂量率：5×10⁷Gy/s），产生了葡萄糖（Gγ = 1×7；Ge = 0×63）和5-脱氧木糖己二醛糖（Gγ = 0×21；Ge = 0×05）。在pH值为5×0或5×5时进行缓冲并辐照会导致这些单体产物，特别是脱氧化合物的生成增加。添加越来越多的牛血清白蛋白或卵清蛋白（10 - 30mg/ml）并辐照，产物产率会显著降低。当在γ或电子辐照过程中存在抹香鲸肌红蛋白时，葡萄糖生成的减少不太明显。然而，在加入约10mg/ml的混合肌红蛋白时，5-脱氧木糖己二醛糖的G值在γ辐照下增加了45%，在电子辐照下增加了70%。肌红蛋白浓度的进一步增加导致脱氧产物的产率逐渐降低。观察到的效应可以用水自由基的清除以及添加的底物与糖自由基的相互作用来解释。乳化脂质（棕榈酸甲酯或三油酸甘油酯）不影响辐射诱导的海藻糖产物的形成。
