Blieva R K
Mikrobiologiia. 1982 Nov-Dec;51(6):945-9.
The production of exohydrolases (alpha-amylase and pectinase) by fungi belonging to the genus Aspergillus was studied in the course of batch cultivation and, if immobilized cells were used, in the semicontinuous regime of growth. The cells were immobilized on a fixed filtering plate and on floating, in the growth medium, polyhedrons. Such a cultivation of immobilized microbial cells in the semicontinuous regime of growth on submerged polyhedrons freely floating in the nutrient medium makes it possible to cultivate the cells for 1.5 months with the active production of exocellular hydrolases. Under these conditions, Aspergillus oryzae 3-9-15 produces more alpha-amylase and A. awamori synthesizes more pectinases.
在分批培养过程中研究了曲霉属真菌产生外切水解酶（α-淀粉酶和果胶酶）的情况，若使用固定化细胞，则在半连续生长模式下进行研究。细胞被固定在固定的过滤板上以及生长培养基中漂浮的多面体上。在营养培养基中自由漂浮的浸没多面体上以半连续生长模式对固定化微生物细胞进行这样的培养，使得能够在细胞外水解酶的活性产生的情况下培养细胞1.5个月。在这些条件下，米曲霉3-9-15产生更多的α-淀粉酶，泡盛曲霉合成更多的果胶酶。
