Seidel G
Arch Geschwulstforsch. 1982;52(6):451-8.
Using the model of chemically induced leukemogenesis of mice by N-nitroso-N-methyl urea (NMU) studies were done to find out the primary point of attack of the chosen carcinogen on cellular level. After application of 14C-NMU (XVII X AKR) F1 hybrid mice were killed at different times, and the 14C activity was determined in various organs by scintillation counting and autoradiography. Contrary to expectations, the bone marrow showed a significantly higher activity than the thymus, which is supposed to be the target organ for lymphatic leukemogenesis. The specificity of the enrichment of 14C activity in bone marrow could be assured by comparative proliferation studies. The autoradiographic results favor the lymphatic cells of bone marrow as target for NMU. The target cell problem is discussed in respect to thymectomy and recent results on nude mice. With high probability the thymus is not essential for lymphatic leukemogenesis and, consequently, is not the target organ.
利用N-亚硝基-N-甲基脲（NMU）诱导小鼠化学性白血病的模型，开展研究以找出所选致癌物在细胞水平上的主要攻击点。给（XVII X AKR）F1杂交小鼠应用14C-NMU后，在不同时间处死小鼠，并通过闪烁计数和放射自显影法测定各器官中的14C活性。与预期相反，骨髓显示出的活性显著高于胸腺，而胸腺被认为是淋巴性白血病发生的靶器官。通过比较增殖研究可以确定骨髓中14C活性富集的特异性。放射自显影结果表明，骨髓中的淋巴细胞是NMU的靶细胞。结合胸腺切除术及近期关于裸鼠的研究结果对靶细胞问题进行了讨论。胸腺极有可能对于淋巴性白血病的发生并非必不可少，因此不是靶器官。
