Soman S, Kaplow L S
J Immunol Methods. 1980;32(3):215-21. doi: 10.1016/0022-1759(80)90187-8.
Monocyte contamination in mononuclear concentrates prepared by Ficoll-Hypaque (F-H) centrifugation was assessed by the following criteria: (a) morphology (Wright-Giemsa stain), (b) function (phagocytosis), and (c) cytochemical staining (non-specific esterase both manually and by flow cytophotometry). Studies on F-H preparations from blood samples obtained from 50 randomly selected patients irmined by functional and cytochemical staining. Monocyte counts determined by flow cytophotometric studies of cells stained in suspension for esterase were very similar to those based on phagocytosis and manual cytochemical staining technics. It is concluded that: (1) manual Wright-Giemsa staining alone is not adequate to accurately quantitate monocyte populations in F-H concentrates, and (2) cytochemical staining and flow cytophotometry can be used for rapid and accurate quantitation of monocyte contamination in F-H mononuclear concentrates.
通过以下标准评估用聚蔗糖-泛影葡胺（F-H）离心法制备的单核细胞浓缩物中的单核细胞污染情况：（a）形态学（瑞氏-吉姆萨染色），（b）功能（吞噬作用），以及（c）细胞化学染色（手工和流式细胞光度法检测非特异性酯酶）。对从50例随机选择患者的血样中获得的F-H制剂进行功能和细胞化学染色研究。通过流式细胞光度法对悬浮染色细胞进行酯酶研究确定的单核细胞计数与基于吞噬作用和手工细胞化学染色技术确定的计数非常相似。得出以下结论：（1）仅靠手工瑞氏-吉姆萨染色不足以准确量化F-H浓缩物中的单核细胞群体，（2）细胞化学染色和流式细胞光度法可用于快速准确地量化F-H单核细胞浓缩物中的单核细胞污染。
