直接选择和分析降低色氨酸操纵子表达水平的突变事件。
Direct selection and analysis of mutational events which diminish the level of expression of the trp operon.
作者信息
Andrews J C, Somerville R L
出版信息
J Bacteriol. 1980 Jan;141(1):422-6. doi: 10.1128/jb.141.1.422-426.1980.
Abstract
A new procedure was devised for selecting, from lac+ galE strains of Escherichia coli, mutants resistant to galactoside-induced lysis. When applied to trp-lac fusions, our method yields down mutations in the trp promoter.
摘要
设计了一种新方法，用于从大肠杆菌的乳糖利用阳性（lac+）半乳糖表型缺陷（galE）菌株中筛选对半乳糖苷诱导裂解具有抗性的突变体。当应用于色氨酸-乳糖操纵子融合体时，我们的方法会导致色氨酸启动子发生负向突变。
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