背靠背放射自显影术：一种用于光学显微镜放射自显影片超微结构分析的方法。

Back to back autoradiography: a method for ultrastructural analysis of light microscopic autoradiographs.

作者信息

Kaplan N B, Smith L J, Brody J S

出版信息

J Histochem Cytochem. 1980 Mar;28(3):276-8. doi: 10.1177/28.3.6986437.

DOI:10.1177/28.3.6986437

Abstract

A method for studying radiolabeled cells is described that combines the simplicity of light microscopic autoradiography with the high resolution of electron microscopy. Serial thin (600 A) and thick (1 micron) sections are placed on Formvar-coated slot grids and glass slides, respectively. Labeled cells are visualized on the thick section by autoradiography and may then be studied in the electron microscope by locating the corresponding fields on the grid. This technique permits accurate ultrastructural identification and analysis of radiolabeled cells, yet avoids the need for electron microscopic autoradiography.

摘要

本文描述了一种研究放射性标记细胞的方法，该方法将光学显微镜放射自显影的简便性与电子显微镜的高分辨率相结合。将连续的薄切片（600埃）和厚切片（1微米）分别置于福尔马林中涂覆的槽形网格和载玻片上。通过放射自显影在厚切片上观察标记细胞，然后通过在网格上定位相应区域在电子显微镜下进行研究。该技术允许对放射性标记细胞进行准确的超微结构鉴定和分析，同时避免了电子显微镜放射自显影的需要。