一种将无皱切片安装在福尔马林中涂覆的槽形网格上的简单方法。
A simple procedure for mounting wrinkle-free sections on formvar-coated slot grids.
作者信息
Rowley J C, Moran D T
出版信息
Ultramicroscopy. 1975 Dec;1(2):151-5. doi: 10.1016/s0304-3991(75)80018-0.
PMID:800684
Abstract
This paper describes a simple technique for consistent production of clean, unwrinkled, flat thin (500-1000 A) sections for TEM and thick (1/2-1 micron) sections for HVEM mounted on Formvar-covered slot grids for use in conventional and high voltage electron microscopy. The technique centers around use of a Formvar-covered aluminum supporting rack onto which slot grids that contain sections suspended in water are placed.
摘要
本文介绍了一种简单的技术，可始终如一地制备出清洁、无褶皱、平整的超薄（500 - 1000埃）切片用于透射电子显微镜（TEM）观察，以及制备出较厚（1/2 - 1微米）切片用于高压电子显微镜（HVEM）观察，这些切片安装在覆盖有福尔马林膜的槽形网格上，用于常规和高压电子显微镜检查。该技术的核心是使用一个覆盖有福尔马林膜的铝制支撑架，将装有悬浮在水中的切片的槽形网格放置在该支撑架上。
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