Fisher B, Wolmark N, Saffer E A
J Natl Cancer Inst. 1980 Mar;64(3):579-85.
Investigations were performed to elucidate changes in normal syngeneic recipients after they received tumor-sensitized cells. Transfer of regional or nonregional lymph node cells or spleen cells from tumor-bearing inbred C3HeB/FeJ mice to normal syngeneic recipients caused the recipients to produced bone marrow, which mediated in vitro cytolysis of immunizing tumor target cells. This finding was not entirely limited to transfer of lymphoid cells. The transfer of myeloid cells, granulocytes, and cultured macrophages from tumor-bearing mice also produced cytotoxic cells. The extent of cytotoxicity following cell transfer was related to the duration of tumor growth in cell donors, to the degree of cytotoxicity, and to the number of cells transferred. Both the iv and ip routes were equally effective. Treatment of cells before transfer with trypsin or pronase, mitomycin C, or sublethal irradiation failed to prevent development of cytotoxicity in cells of the recipient, whereas freeze-thawing abolished this event. Serum from the cell recipient could inhibit the cytotoxicity demonstrated by lymphoid cells derived from the recipient or from a tumor-bearing animal. The findings indicate that "information" transferred to normal animals not previously exposed to a tumor results in production of tumor-specific cytotoxic cells in these animals.
开展了多项研究以阐明正常同基因受体在接受肿瘤致敏细胞后的变化。将来自荷瘤近交系C3HeB/FeJ小鼠的区域或非区域淋巴结细胞或脾细胞转移至正常同基因受体，会使受体产生骨髓，该骨髓介导了对免疫肿瘤靶细胞的体外细胞溶解作用。这一发现并不完全局限于淋巴细胞的转移。来自荷瘤小鼠的髓细胞、粒细胞和培养的巨噬细胞的转移也产生了细胞毒性细胞。细胞转移后的细胞毒性程度与细胞供体中肿瘤生长的持续时间、细胞毒性程度以及转移的细胞数量有关。静脉注射和腹腔注射途径同样有效。在转移前用胰蛋白酶或链霉蛋白酶、丝裂霉素C或亚致死剂量照射处理细胞，未能阻止受体细胞产生细胞毒性，而冻融则消除了这一现象。细胞受体的血清可抑制受体或荷瘤动物来源的淋巴细胞所表现出的细胞毒性。这些发现表明，转移至先前未接触过肿瘤的正常动物的“信息”会导致这些动物产生肿瘤特异性细胞毒性细胞。
