两种直接检测血小板相关IgG（PAIgG）的方法在评估免疫性和非免疫性血小板减少症中的比较。

Comparison of two direct assays for platelet-associated IgG (PAIgG) in assessement of immune and nonimmune thrombocytopenia.

作者信息

Kelton J G, Giles A R, Neame P B, Powers P, Hageman N, Hirsch J

出版信息

Blood. 1980 Mar;55(3):424-9.

Abstract

A number of methods have been developed to measure platelet-associated IgG (PAIgG). The antiglobulin consumption assay directly quantitates IgG on the platelet and is sensitive and specific. A fluorescent anti-IgG assay has recently been described and has the advantage of simplicity. We compared the results of these two PAIgG assays in immune and nonimmune thrombocytopenia and nonthrombocytopenic controls. The antiglobulin consumption assay was negative in 61 of 62 and the fluorescent negative in 54 of 62 assays in nonthrombocytopenic controls, and they were negative in 11 of 13 and 8 of 13 assays, respectively, in nonimmune thrombocytopenic patients. The antiglobulin consumption assay was positive in 54 of 58 and the fluorescent positive in 24 of 58 assays of patients with immune thrombocytopenia (ITP and SLE). The overall sensitivity and specificity of the antiglobulin consumption assay was 94% and 95% and of the fluorescent assay was 44% and 82%.

摘要

已经开发出多种方法来测量血小板相关IgG（PAIgG）。抗球蛋白消耗试验可直接定量血小板上的IgG，且灵敏且特异。最近描述了一种荧光抗IgG试验，其优点是操作简单。我们比较了这两种PAIgG试验在免疫性和非免疫性血小板减少症以及非血小板减少症对照中的结果。在非血小板减少症对照中，62次抗球蛋白消耗试验中有61次为阴性，62次荧光试验中有54次为阴性；在非免疫性血小板减少症患者中，13次试验中分别有11次和8次为阴性。在免疫性血小板减少症（ITP和SLE）患者中，58次抗球蛋白消耗试验中有54次为阳性，58次荧光试验中有24次为阳性。抗球蛋白消耗试验的总体敏感性和特异性分别为94%和95%，荧光试验的总体敏感性和特异性分别为44%和82%。