Hector R F, Lyon F L, Domer J E
Infect Immun. 1981 Dec;34(3):844-50. doi: 10.1128/iai.34.3.844-850.1981.
Membrane-mitochondrial (butanol-hot phosphate-buffered saline) and cytosol (soluble cytoplasmic substances) extracts from seven pathogenic species of Candida were used in in vivo and in vitro immunological assays to study antigenic similarities among the strains with respect to C. albicans. Mice were sensitized with C. albicans serotype A for footpad testing or to provide cells for lymphocyte stimulation assays, and guinea pigs were immunized with whole cells or butanol-hot phosphate-buffered saline extracts of C. albicans to obtain antisera for immunodiffusion assays. When extracts from each of the seven species were used in the assays, they consistently segregated, as determined by statistical or subjective analyses, into three groups. Extracts of C. albicans serotype A or B and C. stellatoidea were the most immunologically reactive in all assays, indicating close similarities between those two species, whereas extracts of C. tropicalis and C. parapsilosis elicited only moderate responses. Extracts from C. krusei, C. guilliermondii, and C. pseudotropicalis were hypo- or nonreactive in the assays, indicating a low level of antigenic relatedness to C. albicans.
从七种致病性念珠菌中提取膜-线粒体（丁醇-热磷酸盐缓冲盐水）和胞质溶胶（可溶性细胞质物质）提取物，用于体内和体外免疫测定，以研究这些菌株与白色念珠菌之间的抗原相似性。用A血清型白色念珠菌致敏小鼠用于足垫试验或提供细胞进行淋巴细胞刺激试验，并用白色念珠菌的全细胞或丁醇-热磷酸盐缓冲盐水提取物免疫豚鼠以获得用于免疫扩散试验的抗血清。当在试验中使用这七种念珠菌的每种提取物时，通过统计或主观分析确定，它们始终分为三组。在所有试验中，A或B血清型白色念珠菌和星状念珠菌的提取物免疫反应性最强，表明这两个菌种之间有密切的相似性，而热带念珠菌和近平滑念珠菌的提取物仅引起中等反应。克柔念珠菌、季也蒙念珠菌和伪热带念珠菌的提取物在试验中反应较弱或无反应，表明它们与白色念珠菌的抗原相关性较低。
