Gaidamovich S Y, Melnikova E E, Väänänen P, Penttinen K
Arch Virol. 1980;64(4):339-47. doi: 10.1007/BF01320619.
Optimal conditions of radial haemolysis in gel (HIG) with Chikungunya (Chik) and Japanese encephalitis (JE) viruses were developed and compared with those of rubella virus. Sheep erythrocytes were found preferable for HIG with Chik and JE viruses, unlike rubella virus, where goose erythrocytes gave best results. Rooster erythrocytes were completely negative in the Chik and JE HIG, again differing from rubella virus, which was practically as good with rooster as with goose erythrocytes. Sheep erythrocytes with rubella virus gave incomplete reactions in HIG. Sensitization of erythrocytes with JE and Chik viruses is better, when borate-phosphate, and not phosphate buffer, is used. Optimum pH values for both viruses should be regarded. Sucrose-acetone, freon and borate-saline antigens from infected mice brain may be used for HIG with Chik and JE viruses.
开发了基孔肯雅病毒（Chik）和日本脑炎病毒（JE）在凝胶中进行径向溶血（HIG）的最佳条件，并与风疹病毒的条件进行了比较。发现绵羊红细胞对于Chik和JE病毒的HIG更合适，这与风疹病毒不同，风疹病毒使用鹅红细胞效果最佳。在Chik和JE的HIG中，公鸡红细胞完全呈阴性，这再次与风疹病毒不同，风疹病毒使用公鸡红细胞和鹅红细胞的效果几乎一样好。风疹病毒与绵羊红细胞在HIG中产生不完全反应。当使用硼酸盐 - 磷酸盐而不是磷酸盐缓冲液时，JE和Chik病毒对红细胞的致敏效果更好。应考虑两种病毒的最佳pH值。来自感染小鼠脑的蔗糖 - 丙酮、氟利昂和硼酸盐 - 盐水抗原可用于Chik和JE病毒的HIG。
