单径向溶血技术在登革热和日本脑炎病毒感染血清学诊断中的应用。
The use of the single radial haemolysis technique in the serological diagnosis of dengue and Japanese encephalitis virus infections.
作者信息
Chan Y C, Tan H C, Tan S H, Balachandran K
出版信息
Bull World Health Organ. 1985;63(6):1043-53.
The single radial haemolysis test for the serological diagnosis of suspected dengue and Japanese encephalitis virus infections uses crude virus antigens with a haemagglutinin titre of 1:320 or 1:640. The results, which may be read 3 hours after the addition of a patient's serum, showed a general agreement between this test and haemagglutination-inhibition tests in the number of case diagnoses that were confirmed. The antibody responses of individual patients shown by the two tests, however, were different, which suggests that the two tests may not be measuring the same antibody. The single radial haemolysis test can distinguish between dengue and Japanese encephalitis viruses using specific mouse hyperimmune sera. Tests on a limited number of sera from Japanese encephalitis patients also showed no cross-reactions with dengue virus antigens in those cases having a low-titred but significant fourfold antibody rise to Japanese encephalitis antigen.
用于疑似登革热和日本脑炎病毒感染血清学诊断的单向辐射溶血试验使用血凝素效价为1:320或1:640的粗制病毒抗原。在加入患者血清3小时后即可读取结果，结果表明该试验与血凝抑制试验在确诊病例数上总体一致。然而，两种试验显示的个体患者抗体反应不同，这表明两种试验可能检测的不是同一种抗体。单向辐射溶血试验可使用特异性小鼠超免疫血清区分登革热病毒和日本脑炎病毒。对有限数量的日本脑炎患者血清进行的检测还表明，在那些对日本脑炎抗原有低滴度但显著四倍抗体升高的病例中，与登革热病毒抗原无交叉反应。
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