Harris P, Osborn M, Weber K
J Cell Biol. 1980 Mar;84(3):668-79. doi: 10.1083/jcb.84.3.668.
Eggs of the sea urchin Strongylocentrotus purpuratus were examined by indirect immunofluorescence microscopy for tubulin-containing structures at intervals from fertilization through first cleavage. The staining revealed that the monaster is made up not only of the sperm aster but also of tubulin-staining fibers originating elsewhere in the egg. The monaster does not divide directly but is broken down first before the amphiaster or interphase asters begin to form. The interphase asters reach a peak of development at the streak stage and are in turn broken down before the formation of the mitotic apparatus. The breakdown of the monaster, interphase asters, as well as the asters of the mitotic apparatus proceeds from the cell center or aster centers to the periphery of the cell and is followed by growth of new asters, also proceeding outward from the aster centers. The pattern suggests a transient wavelike movement of some condition, or factor, which favors microtubule depolymerization.
对紫海胆（Strongylocentrotus purpuratus）的卵进行了间接免疫荧光显微镜检查，以观察从受精到第一次卵裂期间含微管蛋白结构的情况。染色显示，单星体不仅由精子星体组成，还由源自卵中其他部位的微管蛋白染色纤维组成。单星体不会直接分裂，而是在双星体或间期星体开始形成之前先分解。间期星体在条纹期达到发育高峰，并在有丝分裂器形成之前依次分解。单星体、间期星体以及有丝分裂器星体的分解从细胞中心或星体中心向细胞周边进行，随后新的星体生长，同样从星体中心向外进行。这种模式表明某种有利于微管解聚的状态或因子存在短暂的波状运动。
