Lipke P N, Ballou C E
J Bacteriol. 1980 Mar;141(3):1170-7. doi: 10.1128/jb.141.3.1170-1177.1980.
Antibodies were raised against Saccharomyces cerevisiae a-cells that had been exposed to the sex pheromone, alpha-factor. After adsorption of the antiserum with diploid cells, antibodies remained that reacted specifically with the mannan from haploid cells. The characteristic determinant was observed in mannan from pheromone-treated a-cells, in mannan from untreated alpha-cells, and at a much lower concentration, in mannan from control a-cells. The antigens from these three mannans appeared to be identical. The determinant was destroyed by mild-acid hydrolysis or periodate oxidation, but not by proteolysis or digestion with exo-alpha-mannanase. Mutants with altered mannan were unable to express the antigen. Complete acid hydrolysates mannan from alpha-factor-treated a-cells contained mannose, glucose, and N-acetylglucosamine. Partial acid hydrolysis, under conditions that destroyed the antigenic determinant, released only mannose and mannobiose. The mannose fraction was labeled to high specific activity during response of a-cells to alpha-factor if radioactive glucose was the carbon source. Neither alpha- not beta-D-mannopyranosyl phosphate was a hapten. The results are consistent with the presence of a haploid-specific antigen containing an acid-labile mannose determinant and show that the amount of this antigen in a-cell mannan is increased in response to alpha-factor.
制备了针对已暴露于性信息素α-因子的酿酒酵母a细胞的抗体。用二倍体细胞吸附抗血清后，仍保留了与单倍体细胞甘露聚糖特异性反应的抗体。在经信息素处理的a细胞的甘露聚糖、未经处理的α细胞的甘露聚糖中，以及在浓度低得多的对照a细胞的甘露聚糖中，均观察到了特征性决定簇。这三种甘露聚糖的抗原似乎是相同的。该决定簇可被温和酸水解或高碘酸盐氧化破坏，但不能被蛋白水解或外切α-甘露聚糖酶消化破坏。甘露聚糖发生改变的突变体无法表达该抗原。来自经α-因子处理的a细胞的甘露聚糖的完全酸水解产物含有甘露糖、葡萄糖和N-乙酰葡糖胺。在破坏抗原决定簇的条件下进行部分酸水解，仅释放出甘露糖和甘露二糖。如果放射性葡萄糖作为碳源，在a细胞对α-因子的反应过程中，甘露糖部分被标记至高比活性。α-和β-D-甘露吡喃糖基磷酸均不是半抗原。结果表明存在一种含有酸不稳定甘露糖决定簇的单倍体特异性抗原，并表明a细胞甘露聚糖中该抗原的量在对α-因子的反应中增加。
