Young M, Davies M J, Bailey D, Gradwell M J, Smestad-Paulsen B, Wold J K, Barnes R M, Hounsell E F
Department of Biochemistry and Molecular Biology, University College London, UK.
Glycoconj J. 1998 Aug;15(8):815-22. doi: 10.1023/a:1006968117252.
Mannans of the yeast Saccharomyces cerevisiae have been implicated as containing the allergens to which bakers and brewers are sensitive and also the antigen recognized by patients with Crohn's disease. A fraction of S. cerevisiae mannan, Sc500, having high affinity for antibodies in Crohn's patients has been characterized by NMR spectroscopy followed by fragmentation using alkaline elimination, partial acid hydrolysis and acetolysis. The released oligosaccharides were separated by gel filtration on a Biogel P4 column and analyzed by fluorescence labeling, HPLC and methylation analysis. The relationship between structure and antigen activity was measured by competitive ELISA. The antigenic activity of the original high molecular weight mannan could be ascribed to terminal Manalpha1-->3Manalpha1-->2 sequences which are rarely found in human glycoproteins but were over-represented in Sc500 compared to other yeast mannans.
酿酒酵母的甘露聚糖被认为含有面包师和酿酒师敏感的过敏原，也是克罗恩病患者所识别的抗原。酿酒酵母甘露聚糖的一部分Sc500对克罗恩病患者的抗体具有高亲和力，已通过核磁共振光谱进行表征，随后通过碱消除、部分酸水解和乙酰解进行片段化。释放的寡糖通过在Biogel P4柱上进行凝胶过滤分离，并通过荧光标记、高效液相色谱和甲基化分析进行分析。通过竞争性酶联免疫吸附测定法测量结构与抗原活性之间的关系。原始高分子量甘露聚糖的抗原活性可归因于末端的Manα1→3Manα1→2序列，这些序列在人类糖蛋白中很少见，但与其他酵母甘露聚糖相比，在Sc500中含量过高。
