Leffler C W, Hessler J R, Terragno N A
Am J Physiol. 1980 Mar;238(3):H282-6. doi: 10.1152/ajpheart.1980.238.3.H282.
Effects of indomethacin upon ventilation-induced pulmonary vasodilation of fetal goats suggest prostaglandins may be important in perinatal transition of the pulmonary circulation. To further test this hypothesis, left pulmonary arterial and pulmonary venous samples were taken before and after ventilation from anesthetized exteriorized fetal (near-term) goats and sheep utilizing, in different animals, either constant or variable left pulmonary blood flow. Characterization and quantification of prostaglandinlike compounds were accomplished utilizing extraction of acidic lipids, thin-layer chromatography, and tissue cascade bracket bioassay. The primary vascular prostaglandinlike material in both fetal and neonatal animals was PGI2-like (PGI). On passage through the fetal lung, concentrations of prostaglandin I- and E-like compounds decreased considerably. After ventilation and ligation of the umbilical cord, concentrations of both PGE2-like (PGE) and PGI in inferior vena caval blood fell, and there was net production of PGI by the newly ventilated lung. Production of PGI2 by newly ventilated lung could provide an important vasodilator influence that would establish and maintain the low pulmonary vascular resistance that is necessary for successful adaptation to extrauterine life.
消炎痛对胎羊通气诱导的肺血管舒张的影响表明，前列腺素可能在围产期肺循环转变中起重要作用。为进一步验证这一假说，在麻醉的体外胎儿（近足月）山羊和绵羊中，利用不同动物的恒定或可变左肺血流量，在通气前后采集左肺动脉和肺静脉样本。通过酸性脂质提取、薄层色谱和组织级联支架生物测定对类前列腺素化合物进行表征和定量。胎儿和新生动物的主要血管类前列腺素物质均为前列环素样物质（PGI）。在通过胎儿肺时，前列腺素I和E样化合物的浓度显著降低。通气和结扎脐带后，下腔静脉血中PGE2样物质（PGE）和PGI的浓度均下降，新通气的肺有PGI的净产生。新通气的肺产生前列环素可提供重要的血管舒张作用，从而建立并维持成功适应宫外生活所需的低肺血管阻力。
