Yoshimoto R, Kondoh N, Isawa M, Hamuro J
Cancer Immunol Immunother. 1987;25(1):25-30. doi: 10.1007/BF00199297.
The possibility that a plant lectin as a carrier protein would specifically activate T cells, resulting in the augmentation of antitumor immunity was investigated. ATF1011, a nonmitogenic lectin for T cells purified from Aloe arborescens Mill, bound equally to normal and tumor cells. ATF1011 binding on the MM102 tumor cell surfaces augmented anti-trinitrophenyl (TNP) antibody production of murine splenocytes when the mice were primarily immunized with TNP-conjugated MM102 tumor cells. The alloreactive cytotoxic T cell response was also augmented by allostimulator cells binding ATF1011 on the cell surfaces. These augmented responses may be assumed to be mediated by the activation of helper T cells recognizing ATF1011 as a carrier protein. Killer T cells were induced against ATF1011 antigen in the H-2 restricted manner using syngeneic stimulator cells bearing ATF1011 on the cell surfaces. When this lectin was administered intralesionally into the tumors, induction of cytotoxic effector cells was demonstrated. These results suggest that intralesionally administered ATF1011 binds to the tumor cell membrane and activates T cells specific for this carrier lectin in situ, which results in the augmented induction of systemic antitumor immunity.
研究了植物凝集素作为载体蛋白特异性激活T细胞从而增强抗肿瘤免疫力的可能性。从库拉索芦荟中纯化得到的对T细胞无促有丝分裂作用的凝集素ATF1011，与正常细胞和肿瘤细胞的结合能力相同。当小鼠初次用三硝基苯（TNP）偶联的MM102肿瘤细胞免疫时，ATF1011与MM102肿瘤细胞表面的结合增强了小鼠脾细胞抗TNP抗体的产生。细胞表面结合有ATF1011的同种异体刺激细胞也增强了同种异体反应性细胞毒性T细胞反应。这些增强的反应可能是由识别ATF1011作为载体蛋白并被激活的辅助性T细胞介导的。使用细胞表面带有ATF1011的同基因刺激细胞，以H-2限制性方式诱导出针对ATF1011抗原的杀伤性T细胞。当将这种凝集素瘤内注射到肿瘤中时，可证明有细胞毒性效应细胞的诱导。这些结果表明，瘤内注射的ATF1011与肿瘤细胞膜结合并原位激活针对这种载体凝集素的T细胞，从而增强全身抗肿瘤免疫力的诱导。
