Hirose W, Kawagoe M, Hara M, Kitani A, Harigai M, Hirose T, Norioka K, Suzuki K, Nakamura H
Endocrinol Jpn. 1986 Aug;33(4):489-96. doi: 10.1507/endocrj1954.33.489.
The influence of bovine thyroglobulin (Tg) and/or staphylococcus aureus cowan I (SAC) on Tg antibody synthesis has been studied using cultures of 8 Hashimoto's and 5 normal peripheral blood lymphocytes (PBL). The detection of Tg antibody in the culture supernatants was performed by sensitive biotin-avidin solid phase enzyme immunoassay. By using this technique, we were able to detect small amounts of Tg antibody synthesized by cultured Hashimoto's PBL responsive to bovine Tg and/or SAC; PBL from three out of eight patients produced increased levels of Tg antibody in the presence of 0.02 microgram/ml bovine Tg. On the other hand, PBL from two other cases among them which were unresponsive to bovine Tg alone became responsive to bovine Tg following simultaneous stimulation with SAC. PBL from the other three cases failed to respond to bovine Tg or simultaneous stimulation with bovine Tg and SAC. The former five patients had serum Tg tanned red cell hemagglutination (TGHA) titers greater than 1:409,600 except in one case and the latter had serum TGHA titers less than 1:12,800. These results indicated the presence of the different functional stages of B cells to produce Tg antibody in the circulation of Hashimoto's patients and suggested that sufficient number of lymphocytes responsive to bovine Tg are present in the circulation of Hashimoto's patients with high titers of serum TGHA.
利用8例桥本氏病患者和5例正常外周血淋巴细胞（PBL）进行培养，研究了牛甲状腺球蛋白（Tg）和/或金黄色葡萄球菌考恩I型（SAC）对Tg抗体合成的影响。通过灵敏的生物素-抗生物素蛋白固相酶免疫测定法检测培养上清液中的Tg抗体。利用该技术，我们能够检测出对牛Tg和/或SAC有反应的培养桥本氏病PBL合成的少量Tg抗体；8例患者中有3例的PBL在存在0.02微克/毫升牛Tg的情况下产生的Tg抗体水平升高。另一方面，其中另外2例对单独牛Tg无反应的病例，在同时用SAC刺激后对牛Tg有反应。另外3例的PBL对牛Tg或牛Tg与SAC的同时刺激无反应。除1例患者外，前5例患者的血清Tg鞣酸红细胞血凝（TGHA）滴度大于1:409,600，后3例患者的血清TGHA滴度小于1:12,800。这些结果表明，桥本氏病患者循环中存在产生Tg抗体的B细胞的不同功能阶段，并提示血清TGHA滴度高的桥本氏病患者循环中存在足够数量的对牛Tg有反应的淋巴细胞。
