Ghafghazi T, McDaniel M L, Lacy P E
Diabetologia. 1980 Mar;18(3):229-32. doi: 10.1007/BF00251921.
CrCl3, 0.25, 0.5, 1.0, and 1.5 mmol/l inhibited glucose-induced insulin secretion in a reversible and dose dependent manner. Cr also inhibited basal secretion of insulin in the presence of 5.5 mmol/l glucose and insulin secretion stimulated by 50 mmol/l K+ or 15 mmol/l L-leucine. When 2 mmol/l theophylline was employed to potentiate the stimulatory effect of 16.5 mmol/l glucose, the inhibitory effect of 1.5 mmol/l Cr was reduced and that of 0.5 mmol/l virtually abolished. A similar reduction in the inhibitory effect of Cr was observed when the medium calcium concentration was increased from 2.5 to 5, 7.5 and 12.5 mmol/l. Cr did not alter the conversion of 14C-glucose to 14CO2 or 45Ca uptake by isolated islets. It is concluded that the inhibitory effect of Cr on insulin secretion may be mediated through interference with an intracellular function of Ca++ in the beta cell.
三氯化铬，浓度为0.25、0.5、1.0和1.5毫摩尔/升时，以可逆且剂量依赖的方式抑制葡萄糖诱导的胰岛素分泌。铬还在5.5毫摩尔/升葡萄糖存在的情况下抑制胰岛素的基础分泌，以及由50毫摩尔/升钾离子或15毫摩尔/升L - 亮氨酸刺激的胰岛素分泌。当使用2毫摩尔/升茶碱来增强16.5毫摩尔/升葡萄糖的刺激作用时，1.5毫摩尔/升铬的抑制作用减弱，而0.5毫摩尔/升铬的抑制作用几乎消失。当培养基钙浓度从2.5毫摩尔/升增加到5、7.5和12.5毫摩尔/升时，观察到铬的抑制作用有类似程度的降低。铬不会改变分离胰岛中14C - 葡萄糖向14CO2的转化或45Ca的摄取。得出的结论是，铬对胰岛素分泌的抑制作用可能是通过干扰β细胞中钙离子的细胞内功能来介导的。
