在保护大肠杆菌免受过氧化氢毒性影响方面，recA+基因产物比过氧化氢酶和超氧化物歧化酶更重要。
The recA+ gene product is more important than catalase and superoxide dismutase in protecting Escherichia coli against hydrogen peroxide toxicity.
作者信息
Carlsson J, Carpenter V S
出版信息
J Bacteriol. 1980 Apr;142(1):319-21. doi: 10.1128/jb.142.1.319-321.1980.
Abstract
Various deoxyribonucleic acid repair-deficient strains of Escherichia coli K-12 were exposed to hydrogen peroxide under anaerobic conling of the strains was determined. The level of catalase, peroxidase, and superoxide dismutase in cell-free extracts of the strains as well as the capacity of intact cells to decompose hydrogen peroxide were assayed, recA strains were more rapidly killed than other strains with deoxyribonucleic acid repair deficiencies. There was no correlation between the killing rate of the strains and the capacity of intact cells to decompose hydrogen peroxide or the level of catalase and superoxide dismutase in cell-free extracts. The level of peroxidase in cell-free extract was too low to be determined.
摘要
将各种大肠杆菌K - 12的脱氧核糖核酸修复缺陷菌株在厌氧条件下暴露于过氧化氢中，测定菌株的存活情况。分析了这些菌株无细胞提取物中过氧化氢酶、过氧化物酶和超氧化物歧化酶的水平，以及完整细胞分解过氧化氢的能力。recA菌株比其他具有脱氧核糖核酸修复缺陷的菌株更快被杀死。菌株的杀灭率与完整细胞分解过氧化氢的能力或无细胞提取物中过氧化氢酶和超氧化物歧化酶的水平之间没有相关性。无细胞提取物中的过氧化物酶水平过低，无法测定。
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