Sohnle P G, Varkey B, Rose H D
J Allergy Clin Immunol. 1980 May;65(5):376-80. doi: 10.1016/0091-6749(80)90216-x.
Lymphocyte function was studied in two patients with multiple relapses of pulmonary blastomycosis following antifungal therapy. Neither patient was anergic to routine delayed hypersensitivity skin testing with common antigens. Both had normal in vitro lymphocyte transformation responses to standard mitogens and common microbial antigens. The ability of lymphocytes from the two patients to respond to antigens of the infecting organism was evaluated using a yeast phase Blastomyces dermatitidis extract in tests of in vitro lymphocyte function. Both patients demonstrated positive responses to this extract in standard assays of two in vitro parameters of lymphocyte function, lymphocyte transformation, and lymphokine production. Therefore if an immunologic defect is responsible for the repeated relapses of treated pulmonary blastomycosis in these two patients, it apparently is not one of deficient lymphocyte responsiveness against the infecting organism, as has been found using similar techniques in patients with disseminated deep infections caused by other fungal organisms.
对两名接受抗真菌治疗后多次复发肺芽生菌病的患者的淋巴细胞功能进行了研究。两名患者对常见抗原的常规迟发型超敏皮肤试验均无反应缺失。两人对标准有丝分裂原和常见微生物抗原的体外淋巴细胞转化反应均正常。在体外淋巴细胞功能测试中，使用皮炎芽生菌酵母相提取物评估了两名患者淋巴细胞对感染病原体抗原的反应能力。在淋巴细胞功能的两个体外参数（淋巴细胞转化和淋巴因子产生）的标准检测中，两名患者对该提取物均表现出阳性反应。因此，如果免疫缺陷是这两名患者经治疗的肺芽生菌病反复复发的原因，那么显然不是针对感染病原体的淋巴细胞反应性不足，在由其他真菌病原体引起的播散性深部感染患者中使用类似技术也发现了这一点。
