皮炎芽生菌酵母相细胞壁的碱溶性水溶性提取物及等电聚焦组分在外周血淋巴细胞转化中的应用。
Use of an alkali-soluble water-soluble extract of Blastomyces dermatitidis yeast-phase cell walls and isoelectrically focused components in peripheral lymphocyte transformations.
作者信息
Hall N K, Deighton F, Larsh H W
出版信息
Infect Immun. 1978 Feb;19(2):411-5. doi: 10.1128/iai.19.2.411-415.1978.
Abstract
An alkali-soluble water-soluble extract of Blastomyces dermatitidis yeast-phase cell walls was tested for its ability to elicit a response in lymphocytes isolated from the peripheral blood of Blastomyces-infected guinea pigs. Sequential preparations of the antigen were reproducible and specific in the in vitro lymphocyte transformation assay. Cross-reactivity of the antigen was not evident in lymphocyte transformation assays on lymphocytes obtained from Histoplasma-infected guinea pigs or from animals sensitized with complete Freund adjuvant. Fractionation of the antigen was accomplished on an isoelectric-focusing column, using a sucrose density gradient support. Components were assayed for activity in skin testing and lymphocyte transformation. Comparison of column fractions to the whole antigen showed greater response to the whole antigen in in vivo and in vitro assays.
摘要
对皮炎芽生菌酵母相细胞壁的碱溶性水溶性提取物进行了测试，以检测其引发从感染皮炎芽生菌的豚鼠外周血中分离的淋巴细胞产生反应的能力。在体外淋巴细胞转化试验中，抗原的连续制剂具有可重复性和特异性。在从感染组织胞浆菌的豚鼠或用完全弗氏佐剂致敏的动物获得的淋巴细胞的淋巴细胞转化试验中，抗原的交叉反应不明显。抗原的分级分离是在等电聚焦柱上进行的，使用蔗糖密度梯度支持物。对各组分进行皮肤试验和淋巴细胞转化活性测定。将柱分级分离物与全抗原进行比较，结果显示在体内和体外试验中，全抗原引起的反应更强。
相似文献
1
Use of an alkali-soluble water-soluble extract of Blastomyces dermatitidis yeast-phase cell walls and isoelectrically focused components in peripheral lymphocyte transformations.皮炎芽生菌酵母相细胞壁的碱溶性水溶性提取物及等电聚焦组分在外周血淋巴细胞转化中的应用。
Infect Immun. 1978 Feb;19(2):411-5. doi: 10.1128/iai.19.2.411-415.1978.
2
In vivo and in vitro cell-mediated immune responses to a cell wall antigen of Blastomyces dermatitidis.针对皮炎芽生菌细胞壁抗原的体内和体外细胞介导免疫反应。
Infect Immun. 1977 Feb;15(2):429-35. doi: 10.1128/iai.15.2.429-435.1977.
3
Isolation of a purified skin test antigen from Blastomyces dermatitidis yeast-phase cell wall.从皮炎芽生菌酵母相细胞壁中分离纯化皮肤试验抗原
Infect Immun. 1976 Sep;14(3):623-5. doi: 10.1128/iai.14.3.623-625.1976.
4
Blastomyces dermatitidis antigen-induced lymphocytes reactivity in human blastomycosis.皮炎芽生菌抗原诱导的人芽生菌病淋巴细胞反应性
Infect Immun. 1981 Aug;33(2):485-90. doi: 10.1128/iai.33.2.485-490.1981.
5
Isolation of skin test-active preparations from yeast-phase cells of Blastomyces dermatitidis.从皮炎芽生菌酵母相细胞中分离皮肤试验活性制剂。
Infect Immun. 1974 Jul;10(1):42-7. doi: 10.1128/iai.10.1.42-47.1974.
6
Detection of antibodies and delayed dermal hypersensitivity with different lots of Blastomyces dermatitidis yeast lysate antigen: stability and specificity evaluations.不同批次皮炎芽生菌酵母裂解物抗原的抗体检测及迟发性皮肤超敏反应：稳定性和特异性评估
Mycoses. 1997 Nov;40(7-8):303-8. doi: 10.1111/j.1439-0507.1997.tb00236.x.
7
8
WI-1, a novel 120-kilodalton surface protein on Blastomyces dermatitidis yeast cells, is a target antigen of cell-mediated immunity in human blastomycosis.WI-1是皮炎芽生菌酵母细胞上一种新的120千道尔顿表面蛋白，是人类芽生菌病中细胞介导免疫的靶抗原。
Infect Immun. 1992 Oct;60(10):4291-300. doi: 10.1128/iai.60.10.4291-4300.1992.
9
Cross-reactivity between antigens of Coccidioides immitis, Histoplasma capsulatum and Blastomyces dermatitidis in lymphocyte transformation assays.在淋巴细胞转化试验中，粗球孢子菌、荚膜组织胞浆菌和皮炎芽生菌抗原之间的交叉反应性。
Infect Immun. 1979 Sep;25(3):932-8. doi: 10.1128/iai.25.3.932-938.1979.
10
Detection of antibody responses and delayed dermal hypersensitivity with Blastomyces dermatitidis yeast and mycelial lysate antigens.用皮炎芽生菌酵母和菌丝体裂解物抗原检测抗体反应和迟发性皮肤超敏反应。
Mycopathologia. 1991 Jun;114(3):137-44. doi: 10.1007/BF00437202.
引用本文的文献
1
Isoelectric focusing and ELISA evaluation of a Blastomyces dermatitidis human isolate.皮炎芽生菌一株人体分离株的等电聚焦和酶联免疫吸附测定评估
Mycopathologia. 2007 Aug;164(2):73-6. doi: 10.1007/s11046-007-9033-8. Epub 2007 Jun 20.
2
Antigens and chemical composition of Blastomyces dermatitidis.皮炎芽生菌的抗原及化学成分
Mycopathologia. 1982 Apr 23;78(1):47-63. doi: 10.1007/BF00436581.
3
Blastomyces dermatitidis antigen-induced lymphocytes reactivity in human blastomycosis.皮炎芽生菌抗原诱导的人芽生菌病淋巴细胞反应性
Infect Immun. 1981 Aug;33(2):485-90. doi: 10.1128/iai.33.2.485-490.1981.
4
The cell wall of fungal human pathogens: its possible role in host-parasite relationships.人类致病真菌的细胞壁：其在宿主-寄生虫关系中的可能作用。
Mycopathologia. 1982 Sep 17;79(3):159-84. doi: 10.1007/BF01837196.
5
Immunohistopathological studies of blastomycosis: the use of labeled specific antigens in a hamster model of disease.芽生菌病的免疫组织病理学研究：在疾病的仓鼠模型中使用标记的特异性抗原。
Mycopathologia. 1984 Mar 15;85(1-2):17-20. doi: 10.1007/BF00436697.
6
WI-1, a novel 120-kilodalton surface protein on Blastomyces dermatitidis yeast cells, is a target antigen of cell-mediated immunity in human blastomycosis.WI-1是皮炎芽生菌酵母细胞上一种新的120千道尔顿表面蛋白，是人类芽生菌病中细胞介导免疫的靶抗原。
Infect Immun. 1992 Oct;60(10):4291-300. doi: 10.1128/iai.60.10.4291-4300.1992.
7
Immunization of mice with an avirulent pseudohyphal form of Cryptococcus neoformans.用新型隐球菌无毒假菌丝形式对小鼠进行免疫接种。
Mycopathologia. 1979 Sep 28;68(3):179-81. doi: 10.1007/BF00578527.
8
Kinetics of lymphocyte transformation in mice immunized with viable avirulent forms of Cryptococcus neoformans.用新型隐球菌活无毒株免疫的小鼠淋巴细胞转化动力学
Infect Immun. 1979 May;24(2):449-53. doi: 10.1128/iai.24.2.449-453.1979.
本文引用的文献
1
A submicrodetermination of glucose.葡萄糖的亚微量测定
J Biol Chem. 1949 Nov;181(1):149-51.
2
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
3
Immunology of the mycoses. I. Depressed lymphocyte transformation in chronic histoplasmosis.真菌病的免疫学。I. 慢性组织胞浆菌病中淋巴细胞转化受抑制
J Immunol. 1968 Feb;100(2):436-43.
4
Isolation of skin test-active preparations from yeast-phase cells of Blastomyces dermatitidis.从皮炎芽生菌酵母相细胞中分离皮肤试验活性制剂。
Infect Immun. 1974 Jul;10(1):42-7. doi: 10.1128/iai.10.1.42-47.1974.
5
Preparative isotachophoretic separation of skin test antigens from blastomycin purified derivative.从放线菌素纯化衍生物中对皮肤试验抗原进行制备性等速电泳分离。
Infect Immun. 1976 Mar;13(3):758-62. doi: 10.1128/iai.13.3.758-762.1976.
6
Isolation of a purified skin test antigen from Blastomyces dermatitidis yeast-phase cell wall.从皮炎芽生菌酵母相细胞壁中分离纯化皮肤试验抗原
Infect Immun. 1976 Sep;14(3):623-5. doi: 10.1128/iai.14.3.623-625.1976.
7
In vivo and in vitro cell-mediated responses in coccidioidomycosis. I. Immumologic responses of persons with primary, asymptomatic infections.球孢子菌病的体内和体外细胞介导反应。I. 原发性无症状感染患者的免疫反应。
Am Rev Respir Dis. 1976 Nov;114(5):937-43. doi: 10.1164/arrd.1976.114.5.937.
8
Spectrum of in vivo and in vitro cell-mediated immune responses in coccidioidomycosis.球孢子菌病中体内和体外细胞介导免疫反应的谱系
Cell Immunol. 1977 Jun 1;31(1):130-41. doi: 10.1016/0008-8749(77)90012-0.
Zotero 插件