Bekker M L, Kaboev O K, Koval'tsova S V
Mol Gen Genet. 1980 Feb;177(3):541-4. doi: 10.1007/BF00271495.
An UV-sensitive yeast mutant, uvs12, with almost unchanged sensitivity to gamma-irradiation and methylmethane sulphonate was obtained. uvs12, non allelic to any of the known UV-sensitive mutants from radI to rad21 is defective in early steps of excision repair. This inference is based on the fact that after 4-5 h post-irradiation incubation unexcised pyrimidine dimers are retained in nuclear DNA, which follows from two independent tests: the retention of UV-endonuclease-sensitive sites and enhanced survival after photoreactivation.
获得了一种对紫外线敏感的酵母突变体uvs12，它对γ射线和甲基磺酸甲酯的敏感性几乎没有变化。uvs12与已知的从rad1到rad21的任何紫外线敏感突变体都不等位，在切除修复的早期步骤中存在缺陷。这一推断基于以下事实：在照射后培养4-5小时后，未切除的嘧啶二聚体保留在核DNA中，这是通过两项独立测试得出的：紫外线内切酶敏感位点的保留和光复活后存活率的提高。
