Grafstrom R H, Tseng B Y, Goulian M
Cell. 1978 Sep;15(1):131-40. doi: 10.1016/0092-8674(78)90089-2.
In the presence of dUTP, net DNA synthesis in vitro is substantially reduced. Small DNA fragments that arise during in vitro DNA synthesis in the presence of dUTP are produced as a result of dUMP incorporation and subsequent post-replication excision repair process initiated by uracil-DNA-glycosylase. The size of the fragments is dependent upon the amount of dUMP incorporated, but unlike the normal 4S intermediates of DNA synthesis, these repair products are not precursors to high molecular weight DNA but are further degraded. The high levels of dUTPase as well as the presence of RNA primers on most nascent DNA pieces (Tseng and Goulian, 1977) suggest that repair of uracil-containing DNA does not contribute to the generation of the small, nascent DNA pieces found during DNA synthesis in this in vitro system.
在存在dUTP的情况下，体外净DNA合成显著减少。在dUTP存在下体外DNA合成过程中产生的小DNA片段，是由于dUMP掺入以及随后由尿嘧啶-DNA-糖基化酶启动的复制后切除修复过程所致。片段的大小取决于掺入的dUMP的量，但与正常的DNA合成4S中间体不同，这些修复产物不是高分子量DNA的前体，而是会进一步降解。dUTPase的高水平以及大多数新生DNA片段上存在RNA引物（曾和古利安，1977年）表明，含尿嘧啶DNA的修复对该体外系统DNA合成过程中发现的小的新生DNA片段的产生没有贡献。
