Scheller R H, Costantini F D, Kozlowski M R, Britten R J, Davidson E H
Cell. 1978 Sep;15(1):189-203. doi: 10.1016/0092-8674(78)90094-6.
Nine cloned repetitive sequences were labeled, strands-separated and individually hybridized with RNA extracted from the nuclei of gastrula stage sea urchin embryos and of adult sea urchin intestine cells. The concentration of transcripts complementary to each cloned sequence was measured by RNA excess hybridization kinetics and by a DNA excess titration method. Transcripts of certain of the repeat families are present at over 100 times the concentration of transcripts of other families in each RNA. The set of repetitive sequence families highly represented in intestine nuclear RNA is different from that highly represented in gastrula nuclear RNA. Together with the results obtained with mature oocyte RNA and presented in the accompanying paper by Costantini et al. (1978), these findings show that quantitative patterns of repetitive sequence representation in RNA are specific to each cell type. Both strands of all of the nine cloned repeats are represented at some level in all the RNAs studied. Usually, though not always, the concentration of transcripts complementary to the two strands of each repeat do not differ by more than a factor of two. The cloned tracers do not react with polysomal messenger RNA, and the nuclear RNA molecules with which they hybridize are many times larger than the repetitive sequences themselves.
九个克隆的重复序列被标记、链分离，并分别与从原肠胚期海胆胚胎细胞核和成年海胆肠细胞中提取的RNA杂交。通过RNA过量杂交动力学和DNA过量滴定法测量与每个克隆序列互补的转录本浓度。在每种RNA中，某些重复家族的转录本浓度比其他家族的转录本浓度高100倍以上。在肠核RNA中高度代表的重复序列家族与在原肠胚核RNA中高度代表的不同。连同用成熟卵母细胞RNA获得的结果以及Costantini等人（1978年）在随附论文中呈现的结果，这些发现表明RNA中重复序列表达的定量模式对每种细胞类型都是特异的。在所有研究的RNA中，九个克隆重复序列的两条链在某种程度上都有表达。通常，虽然并非总是如此，但与每个重复序列的两条链互补的转录本浓度差异不超过两倍。克隆的示踪剂不与多聚核糖体信使RNA反应，与之杂交的核RNA分子比重复序列本身大许多倍。
