Gillette T E, Allansmith M R, Greiner J V, Janusz M
Am J Ophthalmol. 1980 May;89(5):724-30. doi: 10.1016/0002-9394(80)90295-0.
Main and accessory lacrimal tissues from autopsy and biopsy specimens were compared histologically and immunohistologically. Formaldehyde-fixed, paraffin-embedded specimens were studied by light microscopy with hematoxylinand-eosin and PAS staining. Glutaraldehyde-fixed, Epon-embedded specimens were sectioned at 1 micron, stained with alkaline Giemsa, and studied by light microscopy. Specimens fixed in a solution of alcohol and acetic acid were stained by immunofluorescence techniques for lactoferrin, lysozyme, secretory component, and the immunoglobulins IgG, IgA, IgM, IgD, and IgE. The main and the accessory lacrimal tissues were identical histologically and had identical distributions of secretory products and immunoglobulin-containing plasma cells. The finding of myoepithelial cells in 1-micron sections of accessory lacrimal tissue indicates autonomic innervation in that tissue. This finding, in conjunction with the identical immunohistology, indicates a common source for unstimulated and stimulated tears.
对尸检和活检标本中的主泪腺组织和副泪腺组织进行了组织学和免疫组织学比较。用苏木精-伊红染色和PAS染色对甲醛固定、石蜡包埋的标本进行光学显微镜研究。戊二醛固定、环氧树脂包埋的标本切成1微米厚的切片，用碱性吉姆萨染色，然后进行光学显微镜研究。用酒精和醋酸溶液固定的标本采用免疫荧光技术检测乳铁蛋白、溶菌酶、分泌成分以及免疫球蛋白IgG、IgA、IgM、IgD和IgE。主泪腺组织和副泪腺组织在组织学上相同，分泌产物和含免疫球蛋白的浆细胞分布也相同。在副泪腺组织1微米厚的切片中发现肌上皮细胞，表明该组织有自主神经支配。这一发现与相同的免疫组织学结果相结合，表明未受刺激的泪液和受刺激的泪液有共同来源。
