Synthesis of [5,6-3H]arachidonic acid and its use in development of a sensitive assay for prostacyclin synthetase.
作者信息
Tai H H, Hsu C T, Tai C L, Sih C J
出版信息
Biochemistry. 1980 Apr 29;19(9):1989-93. doi: 10.1021/bi00550a040.
PMID:6990984
Abstract
[5,6-3H]arachidonic acid has been prepared by catalytic reduction of eicosa-cis-8,11,14-trien-5-ynoic acid (IV) over Lindlar catalyst. When either [5,6-3H]arachidonic acid or [5,6-3H]PGH2 is converted into PGI2 by swine aortic microsomes, the tritium at C-6 is lost to the medium. Thus, the progress of this enzymic rearrangement may be monitored by following the rate of tritium release. As swine aortic microsomes contain only low levels of cyclooxygenase, it is necessary to fortify the system with ram seminal vesicular microsomes (rich in cyclooxygenase) when [5,6-3H]arachidonic acid is used as the indirect substrate.
摘要
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