正常妊娠和子痫前期妊娠脐动脉微粒体中前列环素合成酶的动力学
Kinetics of prostacyclin synthetase in umbilical artery microsomes from normal and pre-eclamptic pregnancies.
作者信息
Downing I, Shepherd G L, Lewis P J
出版信息
Br J Clin Pharmacol. 1982 Feb;13(2):195-8. doi: 10.1111/j.1365-2125.1982.tb01355.x.
Abstract
1 Prostacyclin synthetase in umbilical artery microsomes obeys Michaelis-Menten kinetics. 2 The maximum velocity (Vmax) of prostacyclin synthetase prepared from normal umbilical arteries was significantly higher than the Vmax of prostacyclin synthetase in umbilical arteries taken from pre-eclamptic pregnancies. 3 The Michaelis-Menten constant (Km) of the two prostacyclin synthetase preparations was significantly different with the synthetase from pre-eclamptic arteries have a higher affinity for substrate. 4 The limiting factor for prostacyclin synthesis is Vmax at high substrate concentrations which appears to be the case in umbilical arteries. However, at low substrate concentration there would be no difference in prostacyclin synthesis by the two forms of prostacyclin synthetase.
摘要
- 脐动脉微粒体中的前列环素合成酶遵循米氏动力学。2. 由正常脐动脉制备的前列环素合成酶的最大反应速度（Vmax）显著高于取自先兆子痫孕妇脐动脉中前列环素合成酶的Vmax。3. 两种前列环素合成酶制剂的米氏常数（Km）有显著差异，先兆子痫动脉中的合成酶对底物具有更高的亲和力。4. 在高底物浓度下，前列环素合成的限制因素是Vmax，脐动脉似乎就是这种情况。然而，在低底物浓度下，两种形式的前列环素合成酶合成前列环素不会有差异。
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