[胚胎发育过程中人类脾脏和胸腺的免疫球蛋白阳性细胞]

[Immunoglobulin-positive cells of the human spleen and thymus in embryogenesis].

作者信息

Chuich N A

出版信息

Biull Eksp Biol Med. 1980;89(1):38-40.

Abstract

The percentage and types of fluorescence of immunoglobulin-positive cells were studied in the spleen and thymus from 32 human fetuses aged from 11 to 32 weeks by indirect immunofluorescence. During embryogenesis the number of immunoglobulin-positive cells in the spleen rises from 13 to 33.7%, while in the thymus the percentage of these cells is 0--2 and does not depend on the period of the fetus development. Differentiated estimation of immunoglobulin-positive cells was performed for the first time during embryogenesis from the types of fluorescence. Thymocytes have solitary points of fluorescence on the surface. Lymphocytes with solitary and then with multiple points covering all the surface and at last with "caps" of fluorescence appear in the spleen during the period of the development. The different density of immunoglobulin receptors on the surface reflects the level of lymphocyte differentiation.

摘要

通过间接免疫荧光法研究了32例11至32周龄人类胎儿脾脏和胸腺中免疫球蛋白阳性细胞的荧光百分比及类型。在胚胎发育过程中，脾脏中免疫球蛋白阳性细胞的数量从13%升至33.7%，而胸腺中这些细胞的百分比为0%-2%，且不依赖于胎儿发育时期。首次在胚胎发育过程中根据荧光类型对免疫球蛋白阳性细胞进行了分化评估。胸腺细胞表面有孤立的荧光点。在发育期间，脾脏中出现了表面有孤立荧光点、随后有多个荧光点覆盖整个表面、最后有荧光“帽”的淋巴细胞。表面免疫球蛋白受体的不同密度反映了淋巴细胞的分化水平。