Khlystova Z S, Shmeleva S P, Riabchikov O P, Chuich N A
Arkh Anat Gistol Embriol. 1982 May;82(5):77-83.
Spleens of more than 100 human fetuses at the age of 5-34 weeks of development have been studied by means of immunofluorescent methods to identify T- and B-lymphocytes. The spleen lymphatic follicles are detected at the 12th-14th weeks of the embryonal development. Within the same range of time in the mononuclear suspension of the organ T- and B-lymphocytes are defined. The critical period is stated when certain density of lymphocytes and the portion of T- and B-lymphocytes is established in the organ and it is not changed up to the end of embryogenesis. After the 22nd week, the reticular stroma of the lymphatic follicle acquires a complex structure. The following zones can be determined in it: the periarterial where thymus-dependent cells are situated, the zone where thymus-independent cells are situated and the marginal zone surrounding the whole lymphatic follicle.
运用免疫荧光法对100多个发育年龄在5至34周的人类胎儿脾脏进行了研究，以识别T淋巴细胞和B淋巴细胞。脾脏淋巴滤泡在胚胎发育的第12至14周被检测到。在同一时间段内，在该器官的单核悬液中确定了T淋巴细胞和B淋巴细胞。确定了关键时期，即在该器官中建立了一定密度的淋巴细胞以及T淋巴细胞和B淋巴细胞的比例，并且直到胚胎发育结束都没有变化。在第22周之后，淋巴滤泡的网状基质获得了复杂的结构。在其中可以确定以下区域：胸腺依赖细胞所在的动脉周围区域、胸腺非依赖细胞所在的区域以及围绕整个淋巴滤泡的边缘区域。
