Tono-Oka T, Nakayama M, Matsumoto S
Immunology. 1980 Apr;39(4):607-13.
Production of the chemotactic factor for granulocytes (CFG) by human mononuclear cells was studied using the agarose plate method. CFG was produced by mononuclear cells placed in an agarose well, and a chemotactic gradient was formed in the surrounding area. The degree of CFG production by mononuclear cells was estimated by the chemotactic response of indicator granulocytes which were placed in separated agarose wells in the same dish. CFG production was observed after 3 h culture in a well where LPS or anti-beta 2 microglobulin serum stimulated mononuclear cells were placed. CFG did not show chemotactic activity for monocytes. PHA, Con A, PWM and Sepharose-Protein A failed to activate mononuclear cells to produce significant amounts of CFG compared with non-stimulated cells. There was a dose-response relationship between the chemotactic activity of the CFG produced and the number of phagocytic mononuclear cells in well culture. Thus it was concluded that the CFG-producing cells are not lymphocytes, but phagocytic mononuclear cells or monocytes.
采用琼脂糖平板法研究了人单核细胞产生粒细胞趋化因子（CFG）的情况。CFG由置于琼脂糖孔中的单核细胞产生，在周围区域形成趋化梯度。单核细胞产生CFG的程度通过置于同一培养皿中单独琼脂糖孔中的指示粒细胞的趋化反应来评估。在放置了LPS或抗β2微球蛋白血清刺激的单核细胞的孔中培养3小时后，观察到了CFG的产生。CFG对单核细胞没有趋化活性。与未刺激的细胞相比，PHA、Con A、PWM和琼脂糖-蛋白A未能激活单核细胞产生大量的CFG。在孔培养中，产生的CFG的趋化活性与吞噬单核细胞的数量之间存在剂量反应关系。因此得出结论，产生CFG的细胞不是淋巴细胞，而是吞噬单核细胞或单核细胞。
