Goto H, Nakamura S
Jpn J Exp Med. 1980 Feb;50(1):35-43.
The liberation of endotoxin from Escherichia coli by the addition of antibiotics was investigated using a new method of limulus test, i.e. dry up method. The addition of the bactericidal antibiotic, aminobenzylpenicillin or streptomycin sulfate, to the suspension of Escherichia coli (about 10(6)/ml) increased the concentration of endotoxin in the suspension about eight to nine times during a 3-hour period after the addition of antibiotic accompanying with the concomitant decrease of viable cell counts. The addition of the bacteriostatic antibiotic, tetracycline hydrochloride, also increased the endotoxin level, but this increase was rather mild. On the other hand, in the case of the addition of polymyxin B sulfate, there was no evident increase of the endotoxin level. This ability of polymyxin, i.e. the ability to suppress the elevation of endotoxin levels, was shown when polymyxin in the concentration of more than 2.5 micrograms/ml was added against about 10(6)/ml Escherichia coli and was also shown by the combinational use of polymyxin with aminobenzylpenicillin or streptomycin. Furthermore, the endotoxin-inactivating ability of polymyxin was shown in the study where extracted lipopolysaccharide was used in place of viable cells.
采用一种新的鲎试剂检测方法，即干燥法，研究了添加抗生素后大肠杆菌内毒素的释放情况。向大肠杆菌悬液（约10⁶/ml）中添加杀菌抗生素氨卞青霉素或硫酸链霉素后，在添加抗生素后的3小时内，悬液中内毒素浓度增加了约八至九倍，同时活菌数随之减少。添加抑菌抗生素盐酸四环素后，内毒素水平也有所升高，但升高幅度较小。另一方面，添加硫酸多粘菌素B时，内毒素水平没有明显升高。当向约10⁶/ml大肠杆菌中添加浓度超过2.5微克/毫升的多粘菌素时，以及多粘菌素与氨卞青霉素或链霉素联合使用时，均可显示多粘菌素抑制内毒素水平升高的能力。此外，在用提取的脂多糖代替活细胞的研究中，显示了多粘菌素的内毒素灭活能力。
