Nguyen-Dinh P, Trager W
Am J Trop Med Hyg. 1980 May;29(3):339-42. doi: 10.4269/ajtmh.1980.29.339.
Three recently isolated African strains of Plasmodium falciparum were tested in vitro for their response to chloroquine. Both the 48-hour method described earlier and a modified 48-hour test were used, yielding comparable results. Strain FCR-7/Kenya, isolated from a clinically chloroquine-resistant case, was more resistant to the drug in vitro than the two other strains (FCR-8/West Africa and FCN-1/Nigeria, both isolated from chloroquine-sensitive cases). Complete inhibition of parasite growth occurred for strain FCR-7/Kenya in a drug concentration range ten times higher than for strains FCR-8/West Africa and FCN-1/Nigeria. In the modified 48-hour test, a lower erythrocyte suspension (2%) allows continuous growth of the parasites over a 48-hour cycle without necessitating change in medium. It thus offers distinct advantages for routine laboratory work as well as for potential field trials.
对最近分离出的三株非洲恶性疟原虫菌株进行了体外氯喹反应测试。采用了之前描述的48小时方法以及一种改良的48小时测试方法，结果相当。从临床氯喹耐药病例中分离出的FCR - 7/肯尼亚菌株在体外比其他两株菌株（FCR - 8/西非和FCN - 1/尼日利亚，均从氯喹敏感病例中分离）对该药物更具耐药性。FCR - 7/肯尼亚菌株在比FCR - 8/西非和FCN - 1/尼日利亚菌株高十倍的药物浓度范围内才会完全抑制寄生虫生长。在改良的48小时测试中，较低的红细胞悬液（2%）可使寄生虫在48小时周期内持续生长，而无需更换培养基。因此，它在常规实验室工作以及潜在的现场试验中都具有明显优势。
