血液谷氨酰胺的酶法微量测定
Enzymic micro-assay for blood glutamine.
作者信息
Stahl A, Frick A, Imler M, Schlienger J L
出版信息
Clin Chem. 1978 Oct;24(10):1730-3.
Blood glutamine is hydrolyzed by glutaminase; the resulting ammonia is dialyzed out and measured by use of a continuous-flow colorimetric microassay. Concentrations in the range of 10 to 1300 micromol of glutamine per liter can be determined in a 40-microliter sample. With slight modifications, higher concentrations, up to 5 mmol/liter, can be measured. Assay conditions and the qualities of the microtechnique were made optimal. Usual values obtained with this method for arterial and venous blood in man are, respectively, 443 +/- 140 and 466 +/- 142 micromol/liter (mean +/- 2 SD). Corresponding values for rats are 320 +/- 92 and 340 +/- 108 micromol/liter. Fasting causes a decreased blood glutamine.
血液中的谷氨酰胺被谷氨酰胺酶水解；产生的氨被透析出来，并使用连续流动比色微量分析法进行测量。在40微升的样本中，可以测定每升谷氨酰胺浓度在10至1300微摩尔的范围。稍作修改后，可以测量高达5毫摩尔/升的更高浓度。测定条件和微量技术的质量已达到最佳。用这种方法测得的人体动脉血和静脉血的正常值分别为443±140和466±142微摩尔/升（平均值±2标准差）。大鼠的相应值为320±92和340±108微摩尔/升。禁食会导致血液中谷氨酰胺减少。
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