Park K Y, Gregory J
Clin Chem. 1980 Jul;26(8):1170-2.
We describe a simple, rapid method for determining urinary oxalate: isolation by precipitation with calcium chloride and conversion to dimethyl oxalate, which then is measured by gas chromatography. To each sample, tracer amounts of 14C-labeled oxalic acid are added, to determine the analytical recovery of urinary oxalate. Analytical recovery of [14C]oxalic acid added to urine specimens ranged from 15 to 95% (mean, 80%), and corrected recovery, based on calculation isotope-dilution techniques, ranged from 98 to 100%. The urinary excretion of oxalic acid by 18 normal men, ages 23 to 43 years, ranged between 9 and 23 mg/24h, with a mean value of 16 mg; that by 68 patients with small-bowel bypass ranged from 60 to 210 mg/24 h, with a mean of 127 mg.
我们描述了一种简单、快速的测定尿草酸的方法：用氯化钙沉淀分离，然后转化为草酸二甲酯，再通过气相色谱法进行测定。向每个样品中加入微量的14C标记草酸，以测定尿草酸的分析回收率。添加到尿液标本中的[14C]草酸的分析回收率在15%至95%之间（平均为80%），基于同位素稀释技术计算的校正回收率在98%至100%之间。18名年龄在23至43岁之间的正常男性的尿草酸排泄量在9至23毫克/24小时之间，平均值为16毫克；68例小肠旁路手术患者的尿草酸排泄量在60至210毫克/24小时之间，平均值为127毫克。
