气相色谱法快速测定尿中草酸（作者译）

[Rapid method for the determination of urinary oxalic acid by gas liquid chromatography (author's transl)].

作者信息

Decoux G, Boujet C, Rual M, Drouet C, Lafond J L, Favier A, Agnius-Delord C

出版信息

Clin Chim Acta. 1982 Apr 8;120(2):207-17. doi: 10.1016/0009-8981(82)90157-7.

Abstract

A rapid method for the determination of urinary oxalic acid by gas-liquid chromatography is described. The procedure involves extraction of oxalate from urine by tetrahydrofuran followed by evaporation to dryness and subsequent with diesterification with the boron-trifluoride propanol. The derivative is extracted with hexane and is detected by FID gas chromatography. Malonic acid is used as internal standard. Analytical recovery ranged from 94 to 105%. The coefficient of variation in replicate aliquots over the entire range is less than 6%. The expected range for our method is calculated to be 44 to 577 mumol of oxalate per 24-h urine.

摘要

描述了一种用气相色谱法快速测定尿草酸的方法。该方法包括用四氢呋喃从尿液中提取草酸盐，然后蒸发至干，随后与三氟化硼丙醇进行二酯化反应。衍生物用己烷萃取，并用氢火焰离子化检测器气相色谱法检测。丙二酸用作内标。分析回收率在94%至105%之间。整个范围内重复等分试样的变异系数小于6%。计算得出我们方法的预期范围为每24小时尿液中草酸盐44至577微摩尔。