Di Corcia A, Samperi R, Vinci G, D'Ascenzo G
Clin Chem. 1982 Jul;28(7):1457-60.
In this assay for oxalate in urine, oxalate is adsorbed from the urine onto graphitized carbon black (Carbopack B). After desorption and removal of the solvent, oxalic acid is gas-chromatographically measured after being derivatized with BF3/methanol. Chromatography is on Carbopack B/polyethylene glycol (Mr 20000), 93.7/6.3 by weight. The lower limit of detection of urinary oxalate is about 3 mg/L (CV less than or equal to 3.6%). A series of oxalate determinations in 24-h urine samples of 15 subjects gave a mean of 47.1 (SD 15.4) mg/24 h, with an analytical recovery of 97.4% (SD 3.0%, range 93.6-102.4%). Total analysis time for one sample is about 2 h.
在该尿液草酸盐检测方法中，草酸盐从尿液中被吸附到石墨化炭黑（Carbopack B）上。解吸并去除溶剂后，草酸经BF3/甲醇衍生化后用气相色谱法进行测定。色谱柱为Carbopack B/聚乙二醇（分子量20000），按重量比为93.7/6.3。尿草酸盐的检测下限约为3 mg/L（变异系数小于或等于3.6%）。对15名受试者的24小时尿液样本进行的一系列草酸盐测定结果显示，平均值为47.1（标准差15.4）mg/24小时，分析回收率为97.4%（标准差3.0%，范围93.6 - 102.4%）。一个样本的总分析时间约为2小时。
