乙醇对分离的肝细胞中谷胱甘肽浓度的影响。
Effect of ethanol on glutathione concentration in isolated hepatocytes.
作者信息
Viña J, Estrela J M, Guerri C, Romero F J
出版信息
Biochem J. 1980 May 15;188(2):549-52. doi: 10.1042/bj1880549.
Abstract
- Ethanol induces a decrease in GSH (reduced glutathione) concentration is isolated hepatocytes. Maximal effects appear at 20 mM-ethanol. The concentration-dependence of this decrease is paralleled by the concentration-dependence of the activity of alcohol dehydrogenase. 2. Pyrazole, a specific inhibitor of alcohol dehydrogenase, prevents the ethanol-induced GSH depletion. 3. Acetaldehyde, above 0.05 mM, also promotes a decrease in GSH concentration in hepatocytes. 4. Disulfiram (0.05 mM), an inhibitor of aldehyde dehydrogenase, potentiates the fall in GSH concentration caused by acetaldehyde. 5. The findings support the hypothesis that acetaldehyde is responsible for the depletion of GSH induced by ethanol. 6. Methionine prevents the effect of alcohol or acetaldehyde on GSH concentration in hepatocytes.
摘要
- 乙醇会导致分离出的肝细胞中谷胱甘肽（还原型谷胱甘肽）浓度降低。最大效应出现在20 mM乙醇浓度时。这种降低的浓度依赖性与乙醇脱氢酶活性的浓度依赖性平行。2. 吡唑是乙醇脱氢酶的特异性抑制剂，可防止乙醇诱导的谷胱甘肽耗竭。3. 乙醛浓度高于0.05 mM时，也会促使肝细胞中谷胱甘肽浓度降低。4. 双硫仑（0.05 mM）是乙醛脱氢酶的抑制剂，会增强乙醛引起的谷胱甘肽浓度下降。5. 这些发现支持乙醛是乙醇诱导谷胱甘肽耗竭的原因这一假说。6. 蛋氨酸可防止酒精或乙醛对肝细胞中谷胱甘肽浓度的影响。
相似文献
1
Effect of ethanol on glutathione concentration in isolated hepatocytes.乙醇对分离的肝细胞中谷胱甘肽浓度的影响。
Biochem J. 1980 May 15;188(2):549-52. doi: 10.1042/bj1880549.
2
Depletion of hepatic glutathione by ethanol occurs independently of ethanol metabolism.乙醇对肝脏谷胱甘肽的消耗独立于乙醇代谢发生。
Alcohol Clin Exp Res. 1988 Apr;12(2):224-8. doi: 10.1111/j.1530-0277.1988.tb00184.x.
3
In vivo potentiation of 1,2-dibromoethane hepatotoxicity by ethanol through inactivation of glutathione-s-transferase.乙醇通过使谷胱甘肽 - S - 转移酶失活在体内增强1,2 - 二溴乙烷的肝毒性。
Chem Biol Interact. 1996 Jan 5;99(1-3):277-88. doi: 10.1016/0009-2797(95)03678-4.
4
Inhibition by ethanol of rat liver plasma membrane (Na+,K+)ATPase: protective effect of S-adenosyl-L-methionine, L-methionine, and N-acetylcysteine.乙醇对大鼠肝细胞膜（Na⁺，K⁺）ATP酶的抑制作用：S-腺苷-L-甲硫氨酸、L-甲硫氨酸和N-乙酰半胱氨酸的保护作用
Toxicol Appl Pharmacol. 1989 Feb;97(2):216-29. doi: 10.1016/0041-008x(89)90327-x.
5
[Effect of alcohol intoxication and aldehyde dehydrogenase inhibitors on lipid peroxidation in rat liver].[酒精中毒和乙醛脱氢酶抑制剂对大鼠肝脏脂质过氧化的影响]
Ukr Biokhim Zh (1999). 1999 Jul-Aug;71(4):79-83.
6
Endogenous acetaldehyde in rats. Effects of exogenous ethanol, pyrazole, cyanamide and disulfiram.大鼠体内的内源性乙醛。外源性乙醇、吡唑、氰胺和双硫仑的影响。
Biochem Pharmacol. 1985 Nov 15;34(22):3979-82. doi: 10.1016/0006-2952(85)90375-2.
7
8
Role of alcohol dehydrogenase activity and the acetaldehyde in ethanol- induced ethane and pentane production by isolated perfused rat liver.乙醇脱氢酶活性及乙醛在乙醇诱导离体灌注大鼠肝脏产生乙烷和戊烷中的作用。
Biochem J. 1982 Jul 15;206(1):153-6. doi: 10.1042/bj2060153.
9
Mechanism of allyl alcohol toxicity and protective effects of low-molecular-weight thiols studied with isolated rat hepatocytes.用分离的大鼠肝细胞研究烯丙醇毒性机制及低分子量硫醇的保护作用。
Toxicol Appl Pharmacol. 1985 Apr;78(2):169-79. doi: 10.1016/0041-008x(85)90281-9.
10
Metadoxine prevents damage produced by ethanol and acetaldehyde in hepatocyte and hepatic stellate cells in culture.美他多辛可预防乙醇和乙醛对培养的肝细胞和肝星状细胞产生的损伤。
Pharmacol Res. 2001 Nov;44(5):431-6. doi: 10.1006/phrs.2001.0883.
引用本文的文献
1
Thiamine Deficiency and Neuroinflammation Are Important Contributors to Alcohol Use Disorder.硫胺素缺乏和神经炎症是酒精使用障碍的重要促成因素。
Pathophysiology. 2025 Jul 4;32(3):34. doi: 10.3390/pathophysiology32030034.
2
Oxidative stress in a cellular model of alcohol-related liver disease: protection using curcumin nanoformulations.酒精性肝病细胞模型中的氧化应激：姜黄素纳米制剂的保护作用
Sci Rep. 2025 Mar 5;15(1):7752. doi: 10.1038/s41598-025-91139-0.
3
N-Acetylcysteine Ineffective in Alleviating Hangover from Binge Drinking: A Clinical Study.N-乙酰半胱氨酸对缓解暴饮后宿醉无效：一项临床研究
Toxics. 2024 Aug 12;12(8):585. doi: 10.3390/toxics12080585.
4
Sex difference in the association between blood alcohol concentration and serum ferritin.血液酒精浓度与血清铁蛋白之间关联的性别差异。
Front Psychiatry. 2023 Jul 21;14:1230406. doi: 10.3389/fpsyt.2023.1230406. eCollection 2023.
5
Metabolic signature of HepaRG cells exposed to ethanol and tumor necrosis factor alpha to study alcoholic steatohepatitis by LC-MS-based untargeted metabolomics.基于 LC-MS 的非靶向代谢组学研究乙醇和肿瘤坏死因子 α 暴露的 HepaRG 细胞的代谢特征与酒精性脂肪性肝炎。
Arch Toxicol. 2023 May;97(5):1335-1353. doi: 10.1007/s00204-023-03470-y. Epub 2023 Feb 24.
6
The potential effects of HECTD4 variants on fasting glucose and triglyceride levels in relation to prevalence of type 2 diabetes based on alcohol intake.基于饮酒情况，HECTD4 变异对空腹血糖和甘油三酯水平与 2 型糖尿病患病率的潜在影响。
Arch Toxicol. 2022 Sep;96(9):2487-2499. doi: 10.1007/s00204-022-03325-y. Epub 2022 Jun 17.
7
Downregulation of Glutathione-Mediated Detoxification Capacity by Binge Drinking Aggravates Acetaminophen-Induced Liver Injury through IRE1α ER Stress Signaling.暴饮导致的谷胱甘肽介导的解毒能力下调通过IRE1α内质网应激信号加重对乙酰氨基酚诱导的肝损伤。
Antioxidants (Basel). 2021 Dec 5;10(12):1949. doi: 10.3390/antiox10121949.
8
Pathophysiological Aspects of Alcohol Metabolism in the Liver.酒精在肝脏中的代谢的病理生理学方面。
Int J Mol Sci. 2021 May 27;22(11):5717. doi: 10.3390/ijms22115717.
9
Dietary iso-α-acids prevent acetaldehyde-induced liver injury through Nrf2-mediated gene expression.膳食异α-酸通过 Nrf2 介导的基因表达预防乙醛诱导的肝损伤。
PLoS One. 2021 Feb 5;16(2):e0246327. doi: 10.1371/journal.pone.0246327. eCollection 2021.
10
Behavioral and Biochemical Effects of N-Acetylcysteine in Zebrafish Acutely Exposed to Ethanol.N-乙酰半胱氨酸对急性乙醇暴露斑马鱼的行为和生化效应。
Neurochem Res. 2018 Feb;43(2):458-464. doi: 10.1007/s11064-017-2442-2. Epub 2017 Dec 1.
本文引用的文献
1
2
Inhibition of hepatic gluconeogenesis by ethanol.乙醇对肝脏糖异生的抑制作用。
Biochem J. 1969 Mar;112(1):117-24. doi: 10.1042/bj1120117.
3
High-yield preparation of isolated rat liver parenchymal cells: a biochemical and fine structural study.大鼠肝脏实质细胞分离的高效制备：生化与精细结构研究
J Cell Biol. 1969 Dec;43(3):506-20. doi: 10.1083/jcb.43.3.506.
4
The redox state of free nicotinamide-adenine dinucleotide in the cytoplasm and mitochondria of rat liver.大鼠肝脏细胞质和线粒体中游离烟酰胺腺嘌呤二核苷酸的氧化还原状态。
Biochem J. 1967 May;103(2):514-27. doi: 10.1042/bj1030514.
5
The kinetics and mechanism of liver alcohol dehydrogenase with primary and secondary alcohols as substrates.以伯醇和仲醇为底物时肝脏乙醇脱氢酶的动力学及作用机制
Biochem J. 1966 Jul;100(1):34-46. doi: 10.1042/bj1000034.
6
Brain aldehyde dehydrogenase. Localization, purification, and properties.脑醛脱氢酶。定位、纯化及性质
J Biol Chem. 1966 Aug 10;241(15):3533-9.
7
Studies on the regulation of glutathione level in rat liver.大鼠肝脏中谷胱甘肽水平调节的研究。
J Biochem. 1974 Jan;75(1):93-103. doi: 10.1093/oxfordjournals.jbchem.a130387.
8
On the oxidation of succinate by parenchymal cells isolated from rat liver.关于从大鼠肝脏分离的实质细胞对琥珀酸盐的氧化作用。
FEBS Lett. 1975 Mar 1;51(1):80-3. doi: 10.1016/0014-5793(75)80858-1.
9
Mechanism of the protective action of cysteine and penicillamine against acetaldehyde-induced mitochondrial injury.半胱氨酸和青霉胺对乙醛诱导的线粒体损伤的保护作用机制。
Biochem Pharmacol. 1976 Oct 1;25(19):2179-85. doi: 10.1016/0006-2952(76)90130-1.
10
The role of methionine in glutathione biosynthesis by isolated hepatocytes.蛋氨酸在分离的肝细胞谷胱甘肽生物合成中的作用。
Biochem Biophys Res Commun. 1977 Aug 22;77(4):1257-64. doi: 10.1016/s0006-291x(77)80115-0.
Zotero 插件