Serwer P
Biochemistry. 1980 Jun 24;19(13):3001-4. doi: 10.1021/bi00554a026.
By use of multiple-concentration agarose gels, the distance migrated by linear, duplex DNA during agarose gel electrophoresis has been measured as a function of agarose concentration (A) and molecular weight of the DNA (Mr) for molecules with an Mr between 1.88 X 10(6) and 110 X 10(6). Conditions for obtaining a linear plot of log Mr as a function of distance migrated (D) have been found for DNA molecules with an Mr between 5.47 X 10(6) and 110 X 10(6); improved conditions for fractionating such molecules are described. The ratio of the D of a DNA with an Mr of 26.5 X 10(6) to the D of a DNA with an Mr of 77 X 10(6) was plotted as a function of A and electrical field (E). By use of A values between 0.075 and 0.70% and a constant E, this plot had a single maximum. The maximal value of the above ratio was a decreasing function of E, and the A at this maximal value was also a decreasing function of E.
通过使用多浓度琼脂糖凝胶，已测量了线性双链DNA在琼脂糖凝胶电泳过程中迁移的距离，该距离是琼脂糖浓度（A）和DNA分子量（Mr）的函数，其中Mr在1.88×10⁶至110×10⁶之间。对于Mr在5.47×10⁶至110×10⁶之间的DNA分子，已找到获得log Mr作为迁移距离（D）函数的线性图的条件；描述了分离此类分子的改进条件。将Mr为26.5×10⁶的DNA的D与Mr为77×10⁶的DNA的D之比绘制为A和电场（E）的函数。使用0.075%至0.70%之间的A值和恒定的E，该图有一个单一的最大值。上述比率的最大值是E的递减函数，此最大值处的A也是E的递减函数。
