脉冲时间和琼脂糖浓度会影响脉冲场凝胶电泳（PFGE）和交变脉冲电场凝胶电泳（FIGE）过程中cccDNA的电泳迁移率[已修正]。
Pulse time and agarose concentration affect the electrophoretic mobility of cccDNA during PFGE and FIGE [corrected].
作者信息
Sobral B W, Atherly A G
机构信息
Department of Genetics, Iowa State University, Ames 50011.
出版信息
Nucleic Acids Res. 1989 Sep 25;17(18):7359-69. doi: 10.1093/nar/17.18.7359.
Abstract
Circular DNAs have been shown to migrate in an unusual manner during field inversion gel electrophoresis (FIGE) and orthogonal field alternating gel electrophoresis (OFAGE). We studied the effect of varying pulse time and agarose concentration on the electrophoretic mobility of supercoiled (ccc) DNAs ranging from 2 kbp to 16 kbp during FIGE and contoured homogeneous electric fields (CHEF). Both supercoiled and linear molecules display a minimum mobility as a function of pulse time in a CHEF apparatus. Linear and cccDNAs of the same size are differently affected by pulse time. Pulse-time dependence was observed for cccDNAs in both systems. Pulse-time dependence in FIGE is very small at a 1.0% agarose concentration, but is pronounced in 0.8% or 1.2% gels.
摘要
环状DNA在脉冲场凝胶电泳（FIGE）和正交交变电场凝胶电泳（OFAGE）过程中已被证明以一种不寻常的方式迁移。我们研究了在FIGE和等高均匀电场（CHEF）过程中，改变脉冲时间和琼脂糖浓度对2kbp至16kbp超螺旋（ccc）DNA电泳迁移率的影响。在CHEF装置中，超螺旋分子和线性分子的迁移率均随脉冲时间呈现出最小值。相同大小的线性和cccDNA受脉冲时间的影响不同。在两个系统中均观察到cccDNA的脉冲时间依赖性。在1.0%琼脂糖浓度下，FIGE中的脉冲时间依赖性非常小，但在0.8%或1.2%的凝胶中则很明显。
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