Shiraishi H, Kataoka M, Morita Y, Umemoto J
Research Laboratory, Maruho Co. Ltd., Osaka, Japan.
Free Radic Res Commun. 1993;19(5):315-21. doi: 10.3109/10715769309056520.
The production of formaldehyde from tris(hydroxymethyl) aminomethane(Tris) by interaction with hydroxyl radicals(.OH) was studied, since the reaction mixture from the Fenton reaction performed in Tris/HCl buffer was found to be color-developed by colorimetric determination of formaldehyde. The absorption spectrum of chromogens was identical to that of authentic formaldehyde. Color development, which required the presence of Tris, hydrogen peroxide and cupric ions in the Fenton reaction mixture, was inhibited by the addition of hydroxyl radical scavengers such as glucose or hyaluronic acid. These results indicated that formaldehyde was produced when Tris interacted with .OH. With structures similar to Tris, Good's buffers were also found to produce formaldehyde by interaction with .OH. Analysis of formaldehyde derived from these buffers may provide a simple and convenient assay for detecting .OH generation. In evaluating effects of .OH on the biological system in Tris/HCl buffer or certain Good's Buffers, .OH loss may be due to interactions of .OH with these buffers. The formaldehyde produced as a result of such interactions may affect biological systems.
研究了三（羟甲基）氨基甲烷（Tris）与羟基自由基（·OH）相互作用产生甲醛的情况，因为在Tris/HCl缓冲液中进行芬顿反应的反应混合物经比色法测定甲醛后发现有显色现象。发色团的吸收光谱与纯甲醛的吸收光谱相同。显色反应需要在芬顿反应混合物中存在Tris、过氧化氢和铜离子，加入羟基自由基清除剂如葡萄糖或透明质酸可抑制显色反应。这些结果表明，Tris与·OH相互作用时会产生甲醛。与Tris结构相似的Good's缓冲液也被发现可通过与·OH相互作用产生甲醛。对源自这些缓冲液的甲醛进行分析可能会提供一种简单便捷的检测·OH生成的方法。在评估·OH对Tris/HCl缓冲液或某些Good's缓冲液中生物系统的影响时，·OH的损失可能是由于·OH与这些缓冲液的相互作用。这种相互作用产生的甲醛可能会影响生物系统。
