Turcotte R
Infect Immun. 1980 Jun;28(3):660-8. doi: 10.1128/iai.28.3.660-668.1980.
Groups of female C57BL/6 and C3H/St mice were inoculated intraperitoneally (i.p.) with 10(9), 10(7), and 10(5) bacilli and into the right hind footpad with 10(7) and 10(5) bacilli of Mycobacterium lepraemurium. The incidence of death from leprosy and the mean survival time of leprous mice were recorded. In addition, the blastogenic responses to the T-cell mitogens phytohemagglutinin and concanavalin A and to the B-cell mitogens lipopolysaccharide and dextran sulfate were evaluated at various times during the course of infection in the spleen and peripheral lymph nodes of mice infected with 10(7) bacilli. When M. lepraemurium was administered i.p., the two strains of mice succumbed to the disease at about the same time, except for the C57BL/6 mice infected with 10(9) bacilli, which died earlier than the C3H/St mice. Moreover, in both strains of mice, a progressive depression of blastogenesis, first to the T-cell mitogens and then to the B-cell mitogens in the spleen, and to the T-cell mitogens in the peripheral lymph nodes, occurred during the course of the infection, whereas the response to the B-cell mitogens in the nodes increased slowly during the advanced stage of the disease. When 10(7) and 10(5) bacilli were injected into the footpad, the C3H/St mice succumbed to the disease at 298 and 344 days, respectively, and the modifications of blastogenesis were similar to those observed in i.p.-infected C3H/St mice. In contrast, the C57BL/6 mice appeared resistant to footpad inoculation of M. lepraemurium, since they lived until the end of the observation period (466 days postinfection) and the depression of blastogenesis was not detectable until 1 year after the infection. Thus, it is concluded that for the C57BL/6 mice (but not for the C3H/St mice), the route of administration of M. lepraemurium can markedly influence the susceptibility or resistance to leprosy.
将雌性C57BL/6和C3H/St小鼠分组，分别腹腔注射10⁹、10⁷和10⁵条麻风杆菌，以及右后足垫注射10⁷和10⁵条鼠麻风杆菌。记录麻风病致死率和患麻风病小鼠的平均存活时间。此外，在感染过程中的不同时间，评估感染10⁷条杆菌的小鼠脾脏和外周淋巴结对T细胞有丝分裂原植物血凝素和刀豆球蛋白A以及对B细胞有丝分裂原脂多糖和硫酸葡聚糖的增殖反应。当腹腔注射鼠麻风杆菌时，除感染10⁹条杆菌的C57BL/6小鼠比C3H/St小鼠死亡更早外，这两种品系的小鼠大约在同一时间死于该病。此外，在感染过程中，两种品系小鼠的增殖反应均逐渐降低，首先是脾脏中对T细胞有丝分裂原的反应，然后是对B细胞有丝分裂原的反应，外周淋巴结中则是对T细胞有丝分裂原的反应降低，而在疾病晚期，淋巴结中对B细胞有丝分裂原的反应缓慢增加。当将10⁷和10⁵条杆菌注射到足垫时，C3H/St小鼠分别在298天和344天死于该病，增殖反应的变化与腹腔感染的C3H/St小鼠中观察到的相似。相比之下，C57BL/6小鼠似乎对足垫接种鼠麻风杆菌具有抗性，因为它们存活至观察期结束（感染后466天），并且直到感染1年后才检测到增殖反应降低。因此，得出结论，对于C57BL/6小鼠（但不是C3H/St小鼠），鼠麻风杆菌的给药途径可显著影响对麻风病的易感性或抗性。
