用荧光激活细胞分选仪分析大鼠造血细胞。II. 末端脱氧核苷酸转移酶阳性细胞的分离。
Analysis of rat hemopoietic cells on the fluorescence-activated cell sorter. II. Isolation of terminal deoxynucleotidyl transferase-positive cells.
作者信息
Goldschneider I, Metcalf D, Mandel T, Bollum F J
出版信息
J Exp Med. 1980 Aug 1;152(2):438-46. doi: 10.1084/jem.152.2.438.
Abstract
A method is described by which highly enriched populations of viable terminal deoxynucleotidyl transferase-positive (TdT+) cells can be isolated from rat bone marrow by use of the fluorescence-activated cell sorter. Such cells have been postulated to be progenitors of thymocytes and, possibly, of B lymphocytes, and may serve as the targets of neoplastic transformation in acute lymphoblastic leukemia. The separation procedure is based on differences in relative low-angle light scatter and relative fluorescence intensity for Thy-1 antigen between TdT+ cells and other lymphohemopoietic cell populations in bone marrow. Simultaneous sorting of bone marrow cells according to these two parameters resulted in a mean 87% purification of TdT+ cells. The morphological characteristics of the isolated TdT+ cells are described at the light and electron miscroscopic levels.
摘要
本文描述了一种方法，通过该方法可利用荧光激活细胞分选仪从大鼠骨髓中分离出高富集的存活末端脱氧核苷酸转移酶阳性（TdT+）细胞群体。这类细胞被推测为胸腺细胞的祖细胞，也可能是B淋巴细胞的祖细胞，并且可能是急性淋巴细胞白血病中肿瘤转化的靶细胞。分离程序基于TdT+细胞与骨髓中其他淋巴造血细胞群体在Thy-1抗原的相对低角度光散射和相对荧光强度上的差异。根据这两个参数对骨髓细胞进行同时分选，TdT+细胞的平均纯化率达到87%。在光学显微镜和电子显微镜水平上描述了分离出的TdT+细胞的形态特征。
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Analysis of rat hemopoietic cells on the fluorescence-activated cell sorter. I. Isolation of pluripotent hemopoietic stem cells and granulocyte-macrophage progenitor cells.利用荧光激活细胞分选仪分析大鼠造血细胞。I. 多能造血干细胞和粒细胞-巨噬细胞祖细胞的分离。
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Fluorescence activated cell sorting.荧光激活细胞分选
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Is terminal deoxynucleotidyl transferase a somatic mutagen in lymphocytes?末端脱氧核苷酸转移酶是淋巴细胞中的体细胞诱变剂吗？
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