Stenson S, Newman R, Freyne P, Rosenthal S
Ophthalmic Surg. 1980 Jun;11(6):395-7.
One hundred consecutive donor corneas which had been preserved in McCarey-Kaufman medium and used in actual keratoplasties were studied. The residual medium and button remnants were cultured for bacterial and fungal growth. Coagulase-positive staphylococci were recovered in three cases, coagulase-negative staphylococcus in one and Rhodotorula sp. in another; no associated postoperative infections were reported, although one patient was regrafted 48 hours after the initial keratoplasty for "graft decompensation"--the initial button was not submitted for reculture. One additional specimen was processed. A cracked vial with clear contained fluid was held for culture; Pseudomonas cepacia was grown from the fluid. Other samples in undamaged vials from the same lot did not show contamination.
对100个连续的供体角膜进行了研究，这些角膜保存在麦卡里 - 考夫曼培养基中并用于实际角膜移植手术。对剩余的培养基和角膜植片残余物进行细菌和真菌生长培养。3例培养出凝固酶阳性葡萄球菌，1例培养出凝固酶阴性葡萄球菌，另1例培养出红酵母属；尽管1例患者在初次角膜移植术后48小时因“移植失代偿”进行了再次移植，但未报告相关的术后感染——初次的角膜植片未送去重新培养。另外处理了1个样本。一个有裂纹且装有清澈液体的小瓶被送去培养；从液体中培养出洋葱伯克霍尔德菌。同一批次未受损小瓶中的其他样本未显示污染。
