A simple embedding technique for monolayer neuronal cultures grown in plastic flasks.

An in situ embedding technique for monolayer cultures for subsequent ultrastructural studies is described. Dehydration is carried out in graded ethanol series without propylene oxide, and Epon is used as a final embedding medium. The advantage of this method is that the plastic flask with the embedded material can be stripped off easily without affecting the monolayer culture and that the plastic does not dissolve in Epon.