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A simple embedding technique for monolayer neuronal cultures grown in plastic flasks.

作者信息

Spoerri P E, Dresp W, Heyder E

出版信息

Acta Anat (Basel). 1980;107(2):221-3. doi: 10.1159/000145245.

DOI:10.1159/000145245
PMID:6996410
Abstract

An in situ embedding technique for monolayer cultures for subsequent ultrastructural studies is described. Dehydration is carried out in graded ethanol series without propylene oxide, and Epon is used as a final embedding medium. The advantage of this method is that the plastic flask with the embedded material can be stripped off easily without affecting the monolayer culture and that the plastic does not dissolve in Epon.

摘要

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A simple embedding technique for monolayer neuronal cultures grown in plastic flasks.
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