从临床标本中分离嗜肺军团菌：肺组织稀释的有益作用。

Isolation of Legionella pneumophila from clinical specimens: salutary effects of lung tissue dilution.

作者信息

Lattimer G L, Rhodes L V, Salventi J F, Cepil B R

出版信息

Am Rev Respir Dis. 1980 Jul;122(1):101-5. doi: 10.1164/arrd.1980.122.1.101.

DOI:10.1164/arrd.1980.122.1.101

Abstract

Diagnosis of legionnaires' disease is seldom confirmed by isolation of Legionella pneumophila. In 2 cases, isolates were obtained using inoculums of diluted lung tissue suspension, even though no growth ocurred when undiluted suspensions were used. It is speculated that tissue dilution decreases the concentration of antimicrobial agents and antibacterial properties associated with lung host defense mechanisms, which allows improved recovery rates. This technique is recommended when attempting isolation of L. pneumophila from clinical specimens.

摘要

军团病很少通过嗜肺军团菌的分离来确诊。在2例病例中，使用稀释的肺组织悬液接种物获得了分离株，尽管使用未稀释的悬液时未出现生长。据推测，组织稀释降低了与肺宿主防御机制相关的抗菌剂浓度和抗菌特性，从而提高了回收率。当试图从临床标本中分离嗜肺军团菌时，推荐使用这种技术。

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