Fitzgeorge R B, Dennis P J
J Hyg (Lond). 1983 Oct;91(2):179-87. doi: 10.1017/s0022172400060186.
The detection of Legionella pneumophila in water via guinea-pig intraperitoneal injection has been compared with direct isolation of these organisms on semiselective BCYE alpha media. Both techniques were of similar sensitivity, detecting as few as 10(2) L. pneumophila in 10 ml of water. However, at this concentration, detection using guinea-pig intraperitoneal injection was via the indirect parameters of antibody production and immunofluorescent microscopy. Isolation of L. pneumophila from guinea-pig tissue for further investigation required greater than 10(7) organisms injected and in this respect is relatively much less sensitive than direct isolation on semiselective BCYE alpha media. Since L. micdadei and L. longbeachae are inhibited by one of the selective supplements used, other supplements and techniques need to be developed in order to avoid possible inhibition of other Legionella species. This work is based on simulated water samples containing strains of L. pneumophila defined in terms of pneumonic Legionnaires' disease in the guinea-pig. As such, this work serves as a guide to technique and the need to test large numbers of naturally infected water samples in parallel by techniques outlined in this work is emphasized.
通过豚鼠腹腔注射法检测水中嗜肺军团菌，并与在半选择性BCYEα培养基上直接分离这些微生物的方法进行了比较。两种技术的灵敏度相似，在10毫升水中能检测到低至10²个嗜肺军团菌。然而，在此浓度下，使用豚鼠腹腔注射法是通过抗体产生和免疫荧光显微镜等间接参数进行检测的。从豚鼠组织中分离嗜肺军团菌以进行进一步研究，需要注射超过10⁷个微生物，在这方面，它比在半选择性BCYEα培养基上直接分离的灵敏度要低得多。由于米克戴德军团菌和长滩军团菌会被所用的一种选择性添加剂抑制，因此需要开发其他添加剂和技术，以避免可能对其他军团菌属的抑制。这项工作基于含有在豚鼠中根据肺炎型军团病定义的嗜肺军团菌菌株的模拟水样。因此，这项工作可作为技术指南，并强调需要按照本工作所述技术对大量自然感染的水样进行并行检测。
