甲醇脱氢酶的辅基。纯化及其某些性质
The prosthetic group of methanol dehydrogenase. Purification and some of its properties.
作者信息
Duine J A, Frank J
出版信息
Biochem J. 1980 Apr 1;187(1):221-6. doi: 10.1042/bj1870221.
Abstract
Methanol dehydrogenases isolated from bacteria belonging to different classes of methylotrophs contain the same prosthetic group. A procedure for its purification from whole cells is given. The reduced and oxidized form of the enzyme from Hyphomicrobium X and those of the isolated group are compared and it is concluded that the latter indeed functions in the enzyme. Further evidence is presented that the prosthetic group is not a pterine or lumazine derivative, but a water-soluble nitrogen-containing quinone.
摘要
从不同类别的甲基营养菌中分离出的甲醇脱氢酶含有相同的辅基。文中给出了从全细胞中纯化该辅基的方法。对来自纤细杆菌X的酶的还原态和氧化态与分离出的辅基进行了比较，得出的结论是，后者确实在该酶中发挥作用。进一步的证据表明，该辅基不是蝶呤或鲁马嗪衍生物，而是一种水溶性含氮醌。
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