革兰氏阴性菌胡萝卜软腐欧文氏菌外膜和内膜中磷脂酰乙醇胺的分布及流动性
Phosphatidylethanolamine distribution and fluidity in outer and inner membranes of the gram-negative bacterium Erwinia carotovora.
作者信息
Shukla S D, Green C, Turner J M
出版信息
Biochem J. 1980 Apr 15;188(1):131-5. doi: 10.1042/bj1880131.
Abstract
- The distribution of phosphatidylethanolamine, the major lipid of Erwinia carotovora, was investigated in intact bacteria, spheroplasts and outer- and inner-membrane preparations, with the amino-group reagent 2,4,6-trinitrobenzenesulphonic acid. Only 4% was found on the external surface of the outer membrane with 30% on the internal surface, whereas the inner membrane had 27 and 38% on its external and internal surfaces respectively. Some comparative studies were made with three other bacteria. 2. The fluidity of the membranes of E. carotovora was studied by using the fluorescent probe 1,6-diphenylhexa-1,3,5-triene. Results were consistent with the hydrocarbon region of the outer membrane bilayer being less fluid than that of the inner one. 3. On the basis of these and other results a model for the outer- and inner-membrane structures of E. carotovora is proposed.
摘要
- 利用氨基试剂2,4,6-三硝基苯磺酸，对胡萝卜软腐欧文氏菌的主要脂质磷脂酰乙醇胺在完整细菌、原生质体以及外膜和内膜制剂中的分布进行了研究。结果发现，只有4%存在于外膜外表面，30%存在于内表面，而内膜的外表面和内表面分别有27%和38%。还对其他三种细菌进行了一些比较研究。2. 通过使用荧光探针1,6-二苯基己-1,3,5-三烯研究了胡萝卜软腐欧文氏菌膜的流动性。结果表明，外膜双层的烃区流动性低于内膜。3. 根据这些及其他结果，提出了胡萝卜软腐欧文氏菌外膜和内膜结构的模型。
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