荧光探针在磷脂 - 甾醇双层膜研究中的应用。
Use of fluorescent probes in the study of phospholipid--sterol bilayers.
作者信息
Wharton S A, De Martinez S G, Green C
出版信息
Biochem J. 1980 Dec 1;191(3):785-90. doi: 10.1042/bj1910785.
Abstract
- The transfer of excitation energy between the fluorescent probes 1,6-diphenylhexa-1,3,5-triene and 12-(9-anthroyl)stearic acid and the cholesterol analogue cholesta-4,6-dien-3-one in phosphatidylcholine liposomes has been investigated. 2. The results indicate that probes and steroid are randomly distributed in the bilayer at steroid concentrations up to 35 mol%. 3. The degree of polarization of diphenylhexatriene fluorescence increases with increasing cholesterol content. Other sterols, differing in structure in the region of the polar group or in the side chain at position-17, produce similar but not identical effects. 4. the results are consistent with the proposal that diphenylhexatriene gives a general picture of the state of the bilayer and that there is no segregation of sterols in liquid-crystalline phosphatidylcholine bilayers.
摘要
- 研究了荧光探针1,6 - 二苯基己-1,3,5 - 三烯、12 -（9 - 蒽酰基）硬脂酸与胆固醇类似物胆甾-4,6 - 二烯-3 - 酮在磷脂酰胆碱脂质体中的激发能量转移。2. 结果表明，在类固醇浓度高达35摩尔%时，探针和类固醇随机分布在双层膜中。3. 二苯基己三烯荧光的偏振程度随胆固醇含量的增加而增加。在极性基团区域或17位侧链结构不同的其他甾醇产生相似但不完全相同的效果。4. 这些结果与以下提议一致：二苯基己三烯给出了双层膜状态的总体情况，并且在液晶磷脂酰胆碱双层膜中不存在甾醇的分离。
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